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| Catalog Number | Size | Price |
|---|---|---|
| LS-B8176-50 | 50 µg (0.25 mg/ml) | $485 |
IHC‑plus™ Polyclonal Rabbit anti‑Human SDC1 / Syndecan 1 / CD138 Antibody (Internal, IHC, WB) LS‑B8176
IHC‑plus™ Polyclonal Rabbit anti‑Human SDC1 / Syndecan 1 / CD138 Antibody (Internal, IHC, WB) LS‑B8176
Note: This antibody replaces LS-C39398
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
CD138 antibody LS-B8176 is an unconjugated rabbit polyclonal antibody to CD138 (SDC1 / Syndecan 1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SDC1 / Syndecan 1 / CD138
Synonyms
SDC1 | CD138 antigen | SDC | SYND1 | Syndecan | Syndecan 1 | Syndecan-1 | CD138 | Syndecan proteoglycan 1
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide derived from an internal region of the human Syndecan-1 protein.
Epitope
Internal
Specificity
Reacts with the human Syndecan-1 protein. On Western blots, a single band at ~90kD representing the glycosylated form of Syndecan-1; occasionally a band of ~31kD representing the unglycosylated form of Syndecan-1 may be observed. Reactivity has been confirmed with human T47D, SW-480 and PC-3 cell lysates by Western blotting and with formalin-fixed, paraffin-embedded (FFPE) human gastric cancer and breast cancer tissues, and mouse and rat stomach, small bowel, and skin tissues by immunohistochemistry.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml; Confirmed with human T47D, SW-480 and PC-3 cell lysates. A single band at ~90kD representing the glycosylated form of Syndecan-1 is observed and occasionally a band of ~31kD representing the unglycosylated form of Syndecan-1 may be observed. Immunohistochemistry (FFPE): 10 ug/ml; For best results heat induced epitope retrieval with EDTA, pH 8.0, is required prior to staining. Confirmed with human gastric cancer and breast cancer tissues, as well as with mouse and rat stomach, small bowel and skin tissues.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SDC1 / Syndecan 1 / CD138
Validation
Anti-Syndecan / CD138 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Anti-Syndecan / CD138 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ SDC1 / Syndecan 1 / CD138 Antibody (Internal) for IHC, WB/Western, ELISA LS-B8176 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
IHC, Flow Cytometry, ELISA
Species:
Rat
Applications:
Western blot
Reactivity:
Rat
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
Date Requested: 4/28/2024
