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IHCPlus™ PLK3 Antibody aa320‑333 Polyclonal IHC,Peptide‑ELISA,WB LS‑B10518

Catalog Number Size Price
LS-B10518-50 50 µg (0.5 mg/ml) $425 
Huma Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-PRK / PLK3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PLK3 antibody (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Huma Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-PRK / PLK3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PLK3 antibody (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ PLK3 Antibody aa320‑333 Polyclonal IHC,Peptide‑ELISA,WB LS‑B10518

Note: This antibody replaces LS-C186580
Antibody:
Goat Polyclonal to Human PLK3
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10518-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human PLK3
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PLK3 antibody LS-B10518 is an unconjugated goat polyclonal antibody to human PLK3. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PLK3
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence PDRLPISSCVTVPD, from the internal region of the protein sequence according to NP_004064.2.
Blocking Peptide
LS-E28067 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10518. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa320-333
Specificity
Human PRK / PLK3
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in lysates of cell line HeLa (calculated MW of 71.6kD according to NP_004064.2). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PLK3
Q9H4B4 NM_004073 NP_004064.2

Validation

Huma Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Huma Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Huma Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-PRK / PLK3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-PRK / PLK3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PRK / PLK3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/23/2019