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| Catalog Number | Size | Price |
|---|---|---|
| LS-B8086-50 | 50 µl | $565 |
IHC‑plus™ Monoclonal Rabbit anti‑Rat Histone H3 Antibody (Acetyl‑Lys9, IHC, IF, WB) LS‑B8086
IHC‑plus™ Monoclonal Rabbit anti‑Rat Histone H3 Antibody (Acetyl‑Lys9, IHC, IF, WB) LS‑B8086
Note: This antibody replaces LS-C36675
Antibody:
Histone H3 Rabbit anti-Rat Monoclonal (Acetyl-Lys9) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Rat, Human, Monkey, Mouse, Zebrafish
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Histone H3 Rabbit anti-Rat Monoclonal (Acetyl-Lys9) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Rat, Human, Monkey, Mouse, Zebrafish
Format:
Unconjugated, Unmodified
Specifications
Description
Histone H3 antibody LS-B8086 is an unconjugated rabbit monoclonal antibody to Histone H3 (Acetyl-Lys9) from rat. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB.
Host
Rabbit
Reactivity
Rat, Human, Monkey, Mouse, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Immunogen
Synthetic peptide (KLH-coupled) corresponding to the N-Terminus of histone H3 in which Lys9 is acetylated. Species sequence Homology: Rat, monkey - 100%.
Epitope
Acetyl-Lys9
Specificity
Detects endogenous levels of histone H3 only when acetylated on Lys9. Does not cross-react with other acetylated histones. Species cross-reactivity: Human and mouse.
Applications
- IHC
- IHC - Paraffin (1:100)
- Immunofluorescence (1:400)
- Western blot (1:1000)
- Immunoprecipitation (1:25)
- Flow Cytometry (1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Western Blot: 1:1000. Immunoprecipitation: 1:25. Immunohistochemistry (paraffin): 1:100. Recommend antigen retrieval by heating in citrate buffer, pH 6.0. Immunofluorescence (IF-IC): 1:400. Flow Cytometry: 1:200.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-Histone H3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Histone H3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
See More About...
LSBio Ratings
IHC-plus™ Histone H3 Antibody (Acetyl-Lys9) for IHC, IF/Immunofluorescence, WB/Western, IP, Flow LS-B8086 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
ICC, Immunofluorescence, Flow Cytometry, ELISA
Species:
Human, Bovine
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Human
Applications:
IHC, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
Date Requested: 4/26/2024
