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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B8486-50 50 µl (1 mg/ml) $425 
Histone H3 Antibody - Anti-Histone H3 antibody IHC of human breast epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml.
Histone H3 Antibody - Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys18) Antibody. The picture on the right is blocked with the synthesized peptide.
Histone H3 Antibody - Western blot analysis of lysates from HeLa cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys18) Antibody. The lane on the right is blocked with the synthesized peptide.
Histone H3 Antibody - Anti-Histone H3 antibody IHC of human breast epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml.
Histone H3 Antibody - Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys18) Antibody. The picture on the right is blocked with the synthesized peptide.
Histone H3 Antibody - Western blot analysis of lysates from HeLa cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys18) Antibody. The lane on the right is blocked with the synthesized peptide.
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IHCPlus™ Histone H3 Antibody Acetyl‑Lys18 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B8486

IHCPlus™ Histone H3 Antibody Acetyl‑Lys18 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B8486

Note: This antibody replaces LS-C120512
Antibody:
Rabbit Polyclonal to Human Histone H3
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8486-50
50 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Histone H3
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Histone H3 antibody LS-B8486 is an unconjugated rabbit polyclonal antibody to Histone H3 from human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Histone H3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:133 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human Histone H3 around the acetylated site of Lys18. (AA Range: 11-60)
Epitope
Acetyl-Lys18
Specificity
Histone H3 (Acetyl-Lys18) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine18.
Usage
Western Blot: Predicted MW 15 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Histone H3 Antibody - Anti-Histone H3 antibody IHC of human breast epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml.
Anti-Histone H3 antibody IHC of human breast epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml.
Anti-Histone H3 antibody IHC of human breast epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml.

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Requested From: United States
Date Requested: 11/15/2019