Human, Mouse, Rat, Guinea pig, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:50)
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
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Human platelet protein.
Recognizes native, full-length human filamin (actin-binding protein) at 270-280kD and 190kD N-terminal calpain cleavage fragment of filamin. Following induction of apoptosis in U937 cells, recognizes 170, 150, and 120kD N-terminal cleavage fragments of the full-length from presumably resulting from cleavage by activated caspase-3. Species cross-reactivity: Chicken, guinea pig, rabbit and rat.
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2000. Immunoprecipitation: 5 ul per 300-500 ul of cell lysate (200-500 ug total protein). Recommended Lysis buffer is PBS, 05% triton X-100 with proteinase inhibitors. Note: For full-length filamin include calpain inhibitors. Incubate for 1 hour at RT or overnight at 4C. For best recovery, use Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody. 4-8% acrylamide gels are recommended for full-length filamin or the 190kD fragment separation and visualization. Immunofluorescence (Frozen): 1:25-1:100. Immunohistochemistry, human-FFPE, 1:50.
0.1% Sodium Azide
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.