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IHCPlus™ F2R / Thrombin Receptor / PAR1 Antibody N‑Terminus Monoclonal ELISA,Flo,IF,IHC LS‑B2651

Catalog Number Size Price
LS-B2651-50 50 µg (0.5 mg/ml) $485 
Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHCPlus™ F2R / Thrombin Receptor / PAR1 Antibody N‑Terminus Monoclonal ELISA,Flo,IF,IHC LS‑B2651

Note: This antibody replaces LS-C38062
Antibody:
Mouse Monoclonal to Human F2R / Thrombin Receptor / PAR1
Application:
IHC, IHC-P, IF, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B2651-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human F2R / Thrombin Receptor / PAR1
Application:
IHC, IHC-P, IF, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
PAR1 antibody LS-B2651 is an unconjugated mouse monoclonal antibody to human PAR1 (F2R / Thrombin Receptor ). Validated for ELISA, Flow, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human F2R / Thrombin Receptor / PAR1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
ATAP2
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1 - 3 µg/ml)
  • Flow Cytometry (10 - 20 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • Western blot
Immunogen
A synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1.
Epitope
N-Terminus
Specificity
Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2.
Usage
Immunohistochemistry: LS-B2651 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2651 was determined to be 10 ug/ml. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 ug/ml. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 ug/ml. ELISA: 0.1-1.0 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About F2R / Thrombin Receptor / PAR1
P25116 NM_001992 NP_001983.2

Validation

Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Requested From: United States
Date Requested: 9/22/2019