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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B3969-50 50 µg (0.5 mg/ml) $425 
F2R / Thrombin Receptor / PAR1 Antibody - Anti-Thrombin Receptor antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
F2R / Thrombin Receptor / PAR1 Antibody - F2R / Thrombin Receptor / PAR1 antibody (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
F2R / Thrombin Receptor / PAR1 Antibody - Staining (0.5 ug/ml) of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
F2R / Thrombin Receptor / PAR1 Antibody - Anti-Thrombin Receptor antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
F2R / Thrombin Receptor / PAR1 Antibody - F2R / Thrombin Receptor / PAR1 antibody (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
F2R / Thrombin Receptor / PAR1 Antibody - Staining (0.5 ug/ml) of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ Polyclonal Goat anti‑Human F2R / Thrombin Receptor / PAR1 Antibody (aa24‑37, IHC, WB) LS‑B3969

IHCPlus™ Polyclonal Goat anti‑Human F2R / Thrombin Receptor / PAR1 Antibody (aa24‑37, IHC, WB) LS‑B3969

Note: This antibody replaces LS-C101307
Antibody:
Goat Polyclonal to Human F2R / Thrombin Receptor / PAR1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3969-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Human F2R / Thrombin Receptor / PAR1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PAR1 antibody LS-B3969 is an unconjugated goat polyclonal antibody to human PAR1 (F2R / Thrombin Receptor) (aa24-37). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human F2R / Thrombin Receptor / PAR1
Synonyms
F2R | CF2R | Coagulation factor II receptor | PAR1 | PAR-1 | Thrombin receptor | TR | HTR | Protease-activated receptor 1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-TRARRPESKATNAT, from the Internal region (near N-Terminus) of the protein sequence according to NP_001983.2.
Blocking Peptide
LS-E27028 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3969. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa24-37
Specificity
Human F2R / Thrombin receptor.
Usage
Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2).
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About F2R / Thrombin Receptor / PAR1
P25116 NM_001992 NP_001983.2

Validation

F2R / Thrombin Receptor / PAR1 Antibody - Anti-Thrombin Receptor antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-Thrombin Receptor antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-Thrombin Receptor antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

LSBio Ratings

IHC-plus™ F2R / Thrombin Receptor / PAR1 Antibody (aa24-37) for IHC, WB/Western LS-B3969 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/13/2020