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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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F2R / Thrombin Receptor / PAR1

coagulation factor II (thrombin) receptor

Thrombin Receptor is a Proteinase-Activated Receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. Thrombin induced RANTES expression through a PAR-1 mediated pathway influences inflammatory cell migration to the synovium in patients with rheumatoid arthritis.

Gene Name: coagulation factor II (thrombin) receptor
Family/Subfamily: GPCR , Proteinase-activated
Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1
Target Sequences: NM_001992 NP_001983.2 P25116

Filtered by: None
Target
F2R / Thrombin Receptor / PAR1 (80)
Reactivity
Human (80)
Mouse (14)
Rat (11)
Chimpanzee (3)
Gibbon (3)
Monkey (14)
Pig (4)
Rabbit (4)
Application
IHC (29)
IHC-P (25)
WB (53)
Flo (9)
ELISA (38)
ICC (6)
IF (9)
Peptide-ELISA (3)
Host
rabbit (58)
mouse (20)
goat (2)
Isotype
IgG (26)
IgG1 (18)
IgG2b,k (1)
Clonality
monoclonal mc (19)
polyclonal pc (61)
Clone
2C5 (1)
5E2E6 (1)
5E2G12 (1)
6A7H10 (3)
ATAP2 (1)
N2-11 (5)
Format
AP Conjugated (4)
APC Conjugated (4)
Azide-free (3)
Biotin Conjugated (8)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (7)
HRP Conjugated (7)
PE Conjugated (4)
Unconjugated (43)
Epitope
N-Terminus (18)
aa35-63 (8)
aa267-316 (4)
aa51-100 (4)
10-90 aa, N-terminal (1)
40-120 aa, N-terminal (1)
Gly17 (1)
Ser42 (1)
aa1-425 (1)
aa10-59 (1)
aa23-72 (1)
aa24-37 (1)
aa27-102 (1)
aa46-82 (1)
Publications
No (80)
Showing 1-25 of 80 results

IHC-plus™ F2R / Thrombin Receptor / PAR1 Antibody (N-Terminus) LS-A2583
Rabbit Polyclonal to Human F2R / Thrombin Receptor / PAR1
Human
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-F2R / Thrombin Receptor / PAR1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Thrombin Receptor antibody IHC of human bone marrow, megakaryocyte. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ F2R / Thrombin Receptor / PAR1 Antibody (N-Terminus, clone ATAP2) LS-B2651
Mouse Monoclonal [clone ATAP2] (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, Flo, IF, IHC, IHC-P
Unconjugated
50µg 0.5mg/ml  $485.00
Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

F2R / Thrombin Receptor / PAR1 Antibody (HRP) LS-C548626
Mouse Monoclonal (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, Flo, IF
HRP Conjugated
100µl  $800.00

F2R / Thrombin Receptor / PAR1 Antibody (Biotin) LS-C528534
Mouse Monoclonal (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, Flo, IF
Biotin Conjugated
100µl  $800.00

F2R / Thrombin Receptor / PAR1 Antibody (FITC) LS-C538486
Mouse Monoclonal (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, Flo, IF
FITC Conjugated
100µl  $800.00

F2R / Thrombin Receptor / PAR1 Antibody (APC) LS-C518600
Mouse Monoclonal (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, Flo, IF
APC Conjugated
100µl  $800.00

F2R / Thrombin Receptor / PAR1 Antibody (PE) LS-C656641
Mouse Monoclonal (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, Flo, IF
PE Conjugated
100µl  $800.00

F2R / Thrombin Receptor / PAR1 Antibody (AP) LS-C510479
Mouse Monoclonal (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, Flo, IF
AP Conjugated
100µl  $800.00

IHC-plus™ F2R / Thrombin Receptor / PAR1 Antibody (aa24-37) LS-B3969
Goat Polyclonal to Human F2R / Thrombin Receptor / PAR1
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-Thrombin Receptor antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Staining (0.5 ug/ml) of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IHC-plus™ F2R / Thrombin Receptor / PAR1 Antibody (aa23-72) LS-B9308
Rabbit Polyclonal (IgG) to Human F2R / Thrombin Receptor / PAR1
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-F2R / Thrombin Receptor / PAR1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Western blot of extracts from Jurkat cells, using PAR1 (Cleaved-Ser42) Antibody. The lane on the right is treated with the synthesized peptide.

F2R / Thrombin Receptor / PAR1 Antibody (clone N2-11) LS-C180113
Mouse Monoclonal [clone N2-11] (IgG1) to Human F2R / Thrombin Receptor / PAR1
Mouse, Human
ELISA, ICC, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $335.00
Immunohistochemistry: Thrombin Receptor Antibody (N2-11) - IHC of Thrombin Receptor on mouse skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Thrombin Receptor Antibody (N2-11) - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

F2R / Thrombin Receptor / PAR1 Antibody (clone N2-11, Biotin) LS-C180673
Mouse Monoclonal [clone N2-11] (IgG1) to Human F2R / Thrombin Receptor / PAR1
Mouse, Human
ELISA, ICC, IHC, IHC-P, WB
Biotin Conjugated
100µl 0.8mg/ml  $365.00
Immunohistochemistry: Thrombin Receptor Antibody (N2-11) - IHC of Thrombin Receptor on mouse skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Thrombin Receptor Antibody (N2-11) - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

F2R / Thrombin Receptor / PAR1 Antibody (clone N2-11, DY488) LS-C180675
Mouse Monoclonal [clone N2-11] (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human, Monkey
ELISA, ICC, IHC, IHC-P, WB
DY488 Conjugated
100µl 1mg/ml  $365.00
Immunohistochemistry: Thrombin Receptor Antibody (N2-11) - IHC of Thrombin Receptor on mouse skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Thrombin Receptor Antibody (N2-11) - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

F2R / Thrombin Receptor / PAR1 Antibody (clone N2-11, DY550) LS-C180677
Mouse Monoclonal [clone N2-11] (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human, Monkey
ELISA, ICC, IHC, IHC-P, WB
DY550 Conjugated
100µl 1mg/ml  $365.00
Immunohistochemistry: Thrombin Receptor Antibody (N2-11) - IHC of Thrombin Receptor on mouse skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Thrombin Receptor Antibody (N2-11) - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

F2R / Thrombin Receptor / PAR1 Antibody (clone N2-11, DY650) LS-C180674
Mouse Monoclonal [clone N2-11] (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human, Monkey
ELISA, ICC, IHC, IHC-P, WB
DY650 Conjugated
100µl 1mg/ml  $365.00
Immunohistochemistry: Thrombin Receptor Antibody (N2-11) - IHC of Thrombin Receptor on mouse skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Thrombin Receptor Antibody (N2-11) - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

F2R / Thrombin Receptor / PAR1 Antibody (aa267-316) LS-C135497
Rabbit Polyclonal to Human F2R / Thrombin Receptor / PAR1
Rabbit, Mouse, Rat, Pig, Human
WB
Unconjugated
100µl 0.5mg/ml  $385.00
F2R / Thrombin Receptor antibody Western blot of U937 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

F2R / Thrombin Receptor / PAR1 Antibody LS-C408856
Rabbit Polyclonal (IgG) to Human F2R / Thrombin Receptor / PAR1
Mouse, Rat, Human
WB
Unconjugated
50µl  $255.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of various cell lines.

F2R / Thrombin Receptor / PAR1 Antibody LS-C405766
Rabbit Polyclonal (IgG) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, IHC, WB
Unconjugated
20µl 0.2mg/ml  $205.00; 60µl 0.2mg/ml  $235.00; 120µl 0.2mg/ml  $295.00; 200µl 0.2mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human colon cancer using F2R Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of paraffin-embedded Human ovarian cancer using F2R Polyclonal Antibody at dilution of 1:40.

F2R / Thrombin Receptor / PAR1 Antibody (FITC) LS-C395810
Rabbit Polyclonal (IgG) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA
FITC Conjugated
50µg 0.25mg/ml  $285.00; 100µg  $385.00

IHC-plus™ F2R / Thrombin Receptor / PAR1 Antibody LS-B14335
Rabbit Polyclonal to Human F2R / Thrombin Receptor / PAR1
Mouse, Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $425.00
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
PAR-1 Antibody is used in Western blot to detect PAR-1 in rat heart (lane 1) and rat brain (lane 2) tissue lysates.

F2R / Thrombin Receptor / PAR1 Antibody (clone 6A7H10) LS-C171423
Mouse Monoclonal [clone 6A7H10] (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, WB
Unconjugated
100µl 1mg/ml  $345.00
Western blot of PAR1 mouse mAb against truncated GST-PAR1 recombinant protein (1).

F2R / Thrombin Receptor / PAR1 Antibody LS-C800382
Rabbit Polyclonal (IgG) to Human F2R / Thrombin Receptor / PAR1
Mouse, Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunofluorescence Image
Western blot Image

IHC-plus™ F2R / Thrombin Receptor / PAR1 Antibody (N-Terminus) LS-A2621
Rabbit Polyclonal to Human F2R / Thrombin Receptor / PAR1
Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-Thrombin Receptor antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

F2R / Thrombin Receptor / PAR1 Antibody (clone 5E2E6) LS-C682557
Mouse Monoclonal [clone 5E2E6] (IgG1) to Human F2R / Thrombin Receptor / PAR1
Human
ELISA, IHC, WB
Unconjugated
100µl  $365.00

F2R / Thrombin Receptor / PAR1 Antibody (N-Terminus) LS-C352181
Rabbit Polyclonal to Human F2R / Thrombin Receptor / PAR1
Human
ICC, IF, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunofluorescent analysis of PAR1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PAR1 expression in PaCa2 (A); HeLa (B); TF1 (C); ECV304 (D) whole cell lysates.
Showing 1-25 of 80 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).