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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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IHCPlus™ EPHA4 / EPH Receptor A4 Antibody C‑Terminus Polyclonal ELISA,IHC LS‑A2840

Catalog Number Size Price
LS-A2840-50 50 µg (1 mg/ml) $425 
Anti-EPHA4 antibody IHC of human glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ EPHA4 / EPH Receptor A4 Antibody C‑Terminus Polyclonal ELISA,IHC LS‑A2840

Antibody:
Rabbit Polyclonal to Human EPHA4 / EPH Receptor A4
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A2840-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human EPHA4 / EPH Receptor A4
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
EPH Receptor A4 antibody LS-A2840 is an unconjugated rabbit polyclonal antibody to EPH Receptor A4 (EPHA4 ) from human, mouse, rat and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human EPHA4 / EPH Receptor A4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (18 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 20 amino acid peptide from C-terminus of human EPHA4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%); Opossum, Platypus, Lizard (95%); Turkey, Chicken (90%).
Blocking Peptide
LS-E28636 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A2840. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human EPHA4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A2840 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2840 was determined to be 18 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EPHA4 / EPH Receptor A4
P54764 NM_004438 NP_004429.1

Validation

Anti-EPHA4 antibody IHC of human glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-EPHA4 antibody IHC of human glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-EPHA4 antibody IHC of human glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 8/23/2019
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