Human Complement C4d

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

SPM545

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Recombinant human C4d protein.

Human Complement C4d

• IHC
• IHC - Paraffin (0.5 - 10 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• ELISA (1 - 2 µg/ml)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d. C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment. The optimal dilution of the Complement 4d antibody for each application should be determined by the researcher.

1X PBS

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.