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Catalog Number Size Price
LS-C27178-0.1 0.1 mg (1 mg/ml) $835 
Antibody

Monoclonal Mouse anti‑Human Complement C4d Antibody (clone 10‑11, IHC, IF, WB) LS‑C27178

Monoclonal Mouse anti‑Human Complement C4d Antibody (clone 10‑11, IHC, IF, WB) LS‑C27178

Antibody:
Complement C4d Mouse anti-Human Monoclonal (10-11) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$835
LS-C27178-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Complement C4d Mouse anti-Human Monoclonal (10-11) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Complement C4d antibody LS-C27178 is an unconjugated mouse monoclonal antibody to human Complement C4d. Validated for ELISA, IF, IHC and WB.
Target
Human Complement C4d
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit, Sheep
Clonality
IgG1 Monoclonal
Clone
10-11
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Native, from human plasma.
Specificity
Recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR]. C4d was accepted in 2003 into the Banff classification for identification of acute AMR. Is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection. Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4ba€™s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ba€™s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Is used to detect the biomarker C4d which has been described as a a€œfootprinta€ of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream. C4 has also been linked to susceptibility to systemic lupus erythematosus and rheumatoid arthritis.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen (1:100 - 1:750)
  • Immunofluorescence (1:250 - 1:600)
  • Western blot
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial (6).
Presentation
BBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/16/2022