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IHCPlus™ CDC25B Antibody aa56‑105 Polyclonal IHC,WB LS‑B931

Catalog Number Size Price
LS-B931-100 100 µl $485 
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ CDC25B Antibody aa56‑105 Polyclonal IHC,WB LS‑B931

Note: This antibody replaces LS-C30342
Antibody:
Rabbit Polyclonal to Human CDC25B
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B931-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CDC25B
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CDC25B antibody LS-B931 is an unconjugated rabbit polyclonal antibody to CDC25B from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CDC25B
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa56-105 of human CDC25B (P30305, NP_068659). Percent identity by BLAST analysis: Human, Gorilla, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%); Monkey, Bat, Rabbit (92%); Mouse, Rat (85%).
Blocking Peptide
LS-E15204 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B931. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa56-105
Specificity
Human CDC25B
Usage
Immunohistochemistry: LS-B931 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B931 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC25B
P30305 NM_021872 NP_068658.1

Validation

Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 9/16/2019