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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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CDC25B

cell division cycle 25B

CDC25B, a CDC25 dual specificity protein phosphatase, has been shown to affect cell cycle regulation by activating cyclin-dependent kinases Cdk1 and Cdk2 during G2 phase. CDC25B also has been shown to possess oncogenic properties and has been implicated in lung, breast, esophagus, stomach, ovarian, and other cancers. Recently CDC25B has been shown to be a steroid receptor coactivator and this function is independent of the protein phosphatase activity in vitro. CDC25B deficient mice are viable but the female mice are sterile due to the arrest of cell division in oocytes. At least five mRNA splice variants have been documented for CDC25B.

Gene Name: cell division cycle 25B
Family/Subfamily: Protein Phosphatase , CDC25
Synonyms: CDC25B, CDC25B phosphatase, Cell division cycle 25B, M-phase inducer phosphatase 2, CDC25HU2
Target Sequences: NM_021872 NP_068658.1 P30305

Filtered by: None
Target
CDC25B (105)
Reactivity
Human (104)
Mouse (29)
Rat (22)
Bovine (4)
Chimpanzee (3)
Dog (8)
Guinea pig (8)
Horse (5)
Monkey (9)
Pig (4)
Rabbit (4)
Application
IHC (44)
IHC-P (19)
WB (101)
ELISA (47)
ICC (4)
IF (14)
IP (2)
Peptide-ELISA (5)
Host
rabbit (102)
mouse (3)
Isotype
IgG (65)
IgG1,k (1)
IgG2b (2)
Clonality
monoclonal mc (4)
polyclonal pc (101)
Clone
7E8 (1)
EPR3459(2) (1)
OTI11C5 (1)
OTI6H9 (1)
Format
AP Conjugated (5)
APC (1)
APC Conjugated (6)
Azide-free (4)
Biotin Conjugated (11)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (11)
HRP Conjugated (11)
PE Conjugated (6)
Unconjugated (53)
Epitope
Ser353 (8)
aa165-194 (8)
aa331-360 (8)
aa377-566 (8)
pSer187 (8)
C-Terminus (5)
pSer323 (5)
N-Terminus (4)
aa301-350 (4)
aa56-105 (4)
pSer353 (3)
260-340 aa (2)
290-370 aa (2)
Internal (2)
aa421-567 (2)
Arg317 (1)
Lys349 (1)
aa119-248 (1)
aa268-317 (1)
aa289-338 (1)
aa319-368 (1)
aa541-559 (1)
pSer149 (1)
pSer151 (1)
Publications
No (105)
Showing 1-25 of 105 results

IHC-plus™ CDC25B Antibody (Internal, clone EPR3459(2)) LS-B6243
Rabbit Monoclonal [clone EPR3459(2)] to Human CDC25B
Human
IHC, IHC-P, IP, WB
Unconjugated
50µl 0.487mg/ml  $425.00
Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.

CDC25B Antibody (260-340 aa) LS-C380653
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Rat, Human
ELISA, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Phospho-Cdc25B (S323) antibody
Western blot of Phospho-Cdc25B (S323) antibody

IHC-plus™ CDC25B Antibody (aa56-105) LS-B931
Rabbit Polyclonal to Human CDC25B
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Human
IHC, IHC-P, WB
Unconjugated
100µl  $485.00
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CDC25B Antibody (aa289-338) LS-C117556
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunofluorescence analysis of HUVEC cells, using CDC25B Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Raw264.7 cells, treated with Hu 2nM 24h, using CDC25B Antibody. The lane on the right is blocked with the synthesized peptide.

CDC25B Antibody (N-Terminus) LS-C29916
Rabbit Polyclonal (IgG) to Human CDC25B
Rabbit, Dog, Guinea pig, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl 0.5mg/ml  $365.00
CDC25B antibody AVARP03041_P050-NP_068659-CDC25B (cell division cycle 25 homolog B (S. pombe)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Daudi Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CDC25B Antibody (aa331-360) LS-C320230
Rabbit Polyclonal (IgG) to Human CDC25B
Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

CDC25B Antibody (aa331-360, Biotin) LS-C254250
Rabbit Polyclonal (IgG) to Human CDC25B
Human
ELISA, IHC, WB
Biotin Conjugated
200µl  $740.00

CDC25B Antibody LS-C498549
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human ovarian cancer using CDC25B Antibody at dilution of 1:100
Immunofluorescent analysis of Hela cells using CDC25B Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CDC25B Antibody LS-C761169
Rabbit Polyclonal to Human CDC25B
Mouse, Human
ICC, IF, IHC, WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of CDC25B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CDC25B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

CDC25B Antibody LS-C417491
Rabbit Polyclonal to Human CDC25B
Human
ICC, IF, IHC, WB
Unconjugated
100µg  $305.00
Western blot of CDC25B expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

CDC25B Antibody (aa319-368) LS-C117853
Rabbit Polyclonal (IgG) to Human CDC25B
Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CDC25B Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using CDC25B Antibody. The picture on the right is blocked with the synthesized peptide.

CDC25B Antibody (290-370 aa) LS-C382584
Rabbit Polyclonal (IgG) to Human CDC25B
Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

CDC25B Antibody (phospho-Ser323) LS-C801073
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

CDC25B Antibody (phospho-Ser353) LS-C808491
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Human, Monkey
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

IHC-plus™ CDC25B Antibody (aa165-194) LS-B9050
Rabbit Polyclonal to Human CDC25B
Human
IHC, IHC-P, WB
Unconjugated
200µl  $425.00
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of Cdc25B Antibody (A166) in A2058 cell line lysates (35 ug/lane). Cdc25B (arrow) was detected using the purified antibody.

IHC-plus™ CDC25B Antibody (aa331-360) LS-B9049
Rabbit Polyclonal to Human CDC25B
Human
IHC, IHC-P, WB
Unconjugated
200µl  $425.00
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of anti-CDC25B Antibody (Cat #7256c) in Ramos cell line lysates (35 ug/lane). CDC25B(arrow) was detected using the purified antibody.

CDC25B Antibody (Lys349) LS-C176399
Rabbit Polyclonal to Human CDC25B
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of CDC25B (K349) pAb in paraffin-embedded human breast tissue.

CDC25B Antibody LS-C664504
Rabbit Polyclonal (IgG) to Human CDC25B
Human
ELISA, IHC, IP, WB
Unconjugated
100µg  $355.00

CDC25B Antibody LS-C405939
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Human
ELISA, IHC, WB
Unconjugated
20µl 0.6mg/ml  $205.00; 60µl 0.6mg/ml  $235.00; 120µl 0.6mg/ml  $295.00; 200µl 0.6mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human thyroid cancer using CDC25B Polyclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human gastric cancer using CDC25B Polyclonal Antibody at dilution of 1:50.

CDC25B Antibody (phospho-Ser353) LS-C198777
Rabbit Polyclonal (IgG) to Human CDC25B
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain, using CDC25B (Phospho-Ser353) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COS7 cells treated with etoposide 25uM 24h, using CDC25B (Phospho-Ser353) Antibody. The lane on the right is blocked with the phospho peptide.

CDC25B Antibody LS-C417492
Rabbit Polyclonal to Human CDC25B
Mouse, Rat, Human
ICC, IF, WB
Unconjugated
100µg  $305.00
Western blot of CDC25B expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

CDC25B Antibody (Internal) LS-C353933
Rabbit Polyclonal to Human CDC25B
Mouse, Rat, Human, Monkey
ICC, IF, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunofluorescent analysis of CDC25B staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of CDC25B expression in THP1 (A); Jurkat (B); mouse kidney (C); mouse brain (D) whole cell lysates.

CDC25B Antibody (260-340 aa) LS-C382586
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

CDC25B Antibody LS-C801584
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

CDC25B Antibody LS-C490164
Rabbit Polyclonal (IgG) to Human CDC25B
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µg  $435.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image
Showing 1-25 of 105 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).