Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc
(tested or 100% immunogen sequence identity)
Also available conjugated with FITC
- Dot Blot
- Applications tested for the base form of this product only
Highly purified IgG and pools of homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Rat immunoglobulins IgG, IgA, IgM, Fc specific.
The defined antibody specificity is directed to the major isotypes of the rat immunoglobulin system, including antibody to common determinants and to class specific determinants as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates. in radioimmunoassay. for the production of immunoconjugates with a selected marker. to prepare immunoaffinity adsorbents by coupling to an artificial carrier. in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent. in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5000.
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitute with distilled water.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.