Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc
(tested or 100% immunogen sequence identity)
Also available Unconjugated
or conjugated with FITC
- IHC - Paraffin
- Dot Blot
- Applications tested for the base form of this product only
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Purified normal IgG, IgA and IgM isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Rat IgG, IgA and IgM, Fc specific.
The reactivity of the antiserum is directed to the Fc subunit of the major immunoglobulin isotypes (classes and subclasses) which expresses strict isotypic (class) specificity. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of rat origin in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure immunoglobulins in rat serum or other body fluids. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bound to Fc receptors on non-lymphoid cells. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS, pH 7.2, No preservatives added
1 ml Distilled water
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.