Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc
(tested or 100% immunogen sequence identity)
Tetramethyl Rhodamine Iso-Thiocyanate.
Also available Unconjugated
or conjugated with FITC
- IHC - Frozen
- Applications tested for the base form of this product only
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Purified polyclonal rat IgG, and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Rat IgG, IgA and IgM, Fc specific.
The reactivity of the antiserum is directed to the Fc subunits of the major isotypes of the rat immunoglobulin system. No reaction is obtained with purified IgG/Fab or any non-Ig protein of rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS, pH 7.2, No preservatives added
1 ml Distilled water
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.