Mouse Ig Kappa Light Chain

Sheep

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with FITC, TRITC.

Purified IgG

Unmodified

A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure.

Free and bound Mouse Ig kappa light chain.

• Immunofluorescence
• ELISA
• Dot Blot

The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of mouse serum or plasma This IgG preparation is intended for use in precipitating (gel diffusion) and non-precipitating antibody-binding immunoassays (indirect immunofluorescence, enzyme immunoassays for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the kappa type as well as free light chains or Bence Jones proteins of the kappa type in serum or other body fluids.. to prepare an insoluble immunoaffinity adsorbent or a solid phase catching antibody reagent by coupling to an artificial carrier. for labeling with a marker of the user's own choice. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.