Target
Human Ig Kappa Light Chain
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Free Human Ig kappa chain.
Usage
The reactivity of the antiserum is directed to the hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is only reacting with Bence Jones proteins as well as free light chains of the kappa type. No reaction is obtained with intact polyclonal or monoclonal immunoglobulins. To detect and identify free light chain type and Bence Jones protein in serum or urine by To detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the diagnosis of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy. In immunoelectrophoresis use 1.2 ul of sample in a well with a diameter of 1 mm against 60 ul antiserum in a 20 mm long channel. Electrophoresis for 1 hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l. in the case 100 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40% of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.
Presentation
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee