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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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HSPG2 / Perlecan Antibody (Domain V, clone A74) LS-C15758

Antibody

HSPG2 / Perlecan Antibody (Domain V, clone A74) LS-C15758

Antibody:
Mouse Monoclonal to Bovine HSPG2 / Perlecan
Application:
IHC, IHC-Fr, WB, IP, ELISA
Reactivity:
Bovine, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$575 
LS-C15758-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Bovine HSPG2 / Perlecan
Application:
IHC, IHC-Fr, WB, IP, ELISA
Reactivity:
Bovine, Human
Format:
Unconjugated, Unmodified

Specifications

Description
Perlecan antibody LS-C15758 is an unconjugated mouse monoclonal antibody to Perlecan (HSPG2) from human and bovine. Validated for ELISA, IHC, IP and WB.

Target
Bovine HSPG2 / Perlecan
Host
Mouse
Reactivity
Bovine, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
A74
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Western blot (1:50)
  • Immunoprecipitation
  • ELISA (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Bovine corneal endothelial cells. Epitope: domain V.
Epitope
Domain V
Specificity
Highly specific for bovine perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: human.
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Can also be used in affinity chromatography, in conjunction with clone 6F165 to separate recombinant domain I from full length perlecan. ELISA: 1:2000. Western Blot: 1:50. Immunohistochemistry: 1:50. Frozen PLP-fixed sections of bovine and human tissues.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPG2 / Perlecan
P98160 NM_005529 NP_005520.4

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Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Human, Mouse, Rat
Range: Positive/Negative
Reactivity: Human
Range: 0.312-20 ng/ml

Requested From: United States
Date Requested: 6/17/2019
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