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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSPG2 / Perlecan Antibody (Domain V, clone A74) LS‑C15758
Perlecan antibody LS-C15758 is an unconjugated mouse monoclonal antibody to Perlecan (HSPG2) from human and bovine. Validated for ELISA, IHC, IP and WB.
Catalog
Size
Price
LS-C15758-100
100 µl (1 mg/ml)
$575
Description
Perlecan antibody LS-C15758 is an unconjugated mouse monoclonal antibody to Perlecan (HSPG2) from human and bovine. Validated for ELISA, IHC, IP and WB.
Target
Bovine HSPG2 / Perlecan
Host
Mouse
Reactivity
Bovine, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [A74]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Western blot (1:50)
  • Immunoprecipitation
  • ELISA (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Bovine corneal endothelial cells. Epitope: domain V.
Epitope
Domain V
Specificity
Highly specific for bovine perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: human.
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Can also be used in affinity chromatography, in conjunction with clone 6F165 to separate recombinant domain I from full length perlecan. ELISA: 1:2000. Western Blot: 1:50. Immunohistochemistry: 1:50. Frozen PLP-fixed sections of bovine and human tissues.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About HSPG2 / Perlecan
P98160 NM_005529 NP_005520.4

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Requested From: United States
Date Requested: 12/19/2018
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