Human HSPG2 / Perlecan
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Paraffin
- IHC - Frozen
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Recombinant Perlecan domain III.
Recognizes domain III of human perlecan. Domain III shares homology with the short arm of laminin A and B chains.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1-2 ug/ml. Immunohistochemistry (Frozen or FFPE): 5-10 ug/ml. Note: Formalin-fixed, paraffin sections require pretreatment with 0.1% protease for 30 minutes at RT prior to the blocking step. Positive control: Human breast, colon, liver, lymph nodes, pancreas and prostate tissues.
PBS, pH 7.4, 0.09% Sodium Azide
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.