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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSPG2 / Perlecan Antibody (clone A7L6) LS‑C15761

Antibody

HSPG2 / Perlecan Antibody (clone A7L6) LS‑C15761

Antibody:
Rat Monoclonal to Mouse HSPG2 / Perlecan
Application:
IHC, IHC-Fr, IF
Reactivity:
Mouse, Human, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$665 
LS-C15761-500
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Mouse HSPG2 / Perlecan
Application:
IHC, IHC-Fr, IF
Reactivity:
Mouse, Human, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Perlecan antibody LS-C15761 is an unconjugated rat monoclonal antibody to Perlecan (HSPG2) from human, mouse, bovine and other species. Validated for IF and IHC.
Target
Mouse HSPG2 / Perlecan
Host
Rat
Reactivity
Mouse, Human, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
A7L6
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen (1 - 2 µg/ml)
  • Immunofluorescence
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
  • Western blot
Immunogen
Murine EHS laminin preparation.
Specificity
Recognizes perlecan. Does not cross-react with laminin, fibronectin or dermatran sulfate proteoglycan. Species cross-reactivity: Human, bovine, pig, mouse.
Usage
Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Immunohistochemistry (Frozen and acid-alcohol fixed): 1-2 ug/ml for 30 minutes at RT. Staining of acid-alcohol-fixed tissues requires antigen unmasking with 15000u/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37?C. Positive control: Tonsil.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPG2 / Perlecan
P98160 NM_005529 NP_005520.4

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Featured Products

Reactivity: Rat
Range: 31.25-2000 pg/ml
Reactivity: Human
Range: 0.2-50 ng/ml
Reactivity: Human
Range: 78-5000 pg/ml
Reactivity: Human
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 7/22/2019
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