Perlecan antibody LS-C15761 is an unconjugated rat monoclonal antibody to Perlecan (HSPG2) from human, mouse, bovine and other species. Validated for IF and IHC.
Mouse HSPG2 / Perlecan
Mouse, Human, Bovine, Pig
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Frozen (1 - 2 µg/ml)
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- IHC - Paraffin
- Western blot
Murine EHS laminin preparation.
Recognizes perlecan. Does not cross-react with laminin, fibronectin or dermatran sulfate proteoglycan. Species cross-reactivity: Human, bovine, pig, mouse.
Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Immunohistochemistry (Frozen and acid-alcohol fixed): 1-2 ug/ml for 30 minutes at RT. Staining of acid-alcohol-fixed tissues requires antigen unmasking with 15000u/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37?C. Positive control: Tonsil.
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.