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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSPD1 / HSP60 Antibody (aa383‑447) LS‑C15681

Antibody

HSPD1 / HSP60 Antibody (aa383‑447) LS‑C15681

Antibody:
Mouse Monoclonal to Human HSPD1 / HSP60
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Hamster, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$650 
LS-C15681-500
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human HSPD1 / HSP60
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Hamster, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
HSP60 antibody LS-C15681 is an unconjugated mouse monoclonal antibody to HSP60 (HSPD1) from human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Target
Human HSPD1 / HSP60
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSPD1 / HSP60 antibody was raised against recombinant HSP60 protein.
Epitope
aa383-447
Specificity
Recognizes only the mammalian hsp60 at 60kD. Does not recognize bacterial hsp60. Epitope maps between aa383-447 of human hsp60. Species cross-reactivity: human, mouse, rat, hamster and chicken. Does not react with bacteria, helminths or spinach.
Usage
Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native): 2 ug/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml for 2 hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: BT474 cells, MAD109 cells and breast carcinoma.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPD1 / HSP60
P10809 NM_002156 NP_002147.2

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Requested From: United States
Date Requested: 7/20/2019
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