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| Catalog Number | Size | Price |
|---|---|---|
| LS-B10529-50 | 50 µg (1 mg/ml) | $555 |
IHC‑plus™ Monoclonal Mouse anti‑Human HSPD1 / HSP60 Antibody (clone 4E101, aa383‑419, IHC, WB) LS‑B10529
IHC‑plus™ Monoclonal Mouse anti‑Human HSPD1 / HSP60 Antibody (clone 4E101, aa383‑419, IHC, WB) LS‑B10529
Note: This antibody replaces LS-C15687
Antibody:
HSPD1 / HSP60 Mouse anti-Human Monoclonal (aa383-419) (4E101) Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken, Bacteria, Plant
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HSPD1 / HSP60 Mouse anti-Human Monoclonal (aa383-419) (4E101) Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken, Bacteria, Plant
Format:
Unconjugated, Unmodified
Specifications
Description
HSP60 antibody LS-B10529 is an unconjugated mouse monoclonal antibody to HSP60 (HSPD1) (aa383-419) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken, Bacteria, Plant
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
4E101
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human Hsp60 produced in E. coli.
Epitope
aa383-419
Specificity
Recognizes human Hsp60 at ~60kD. Epitope: located within aa 383-419 of human Hsp60 (aa 356-393 of the mycobacterial Hsp60). Species Crossreactivity: mouse, rat, bovine, bacteria, C. elegans, chicken, canine, guinea pig, hamster, monkey, porcine, rabbit, plant.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- Flow Cytometry (10 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and FACS. Other applications not tested. Flow Cytometry: 10ug/ml. Optimal dilutions to be determined by researcher. Positive Controls: HeLa Heat Shocked Cell Lysate, Recombinant Human Hsp60 Protein
Presentation
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSPD1 / HSP60
Validation
Anti-HSPD1 / HSP60 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSPD1 / HSP60 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HSPD1 / HSP60 Antibody (aa383-419, clone 4E101) for IHC, WB/Western, Flow, ELISA LS-B10529 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
