Human HSPD1 / HSP60

Mouse

Human (tested or 100% immunogen sequence identity)

Bacteria, Helminth, Spinach

IgG1,k Recombinant Monoclonal

rGROEL/780

Unconjugated

Protein A/G purified

Unmodified

Recombinant full-length human HSP60 protein. Antigen Molecular Weight: 60 kDa

Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone rGROEL/780, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

• IHC - Paraffin (1:100)
• Western blot (0.25 - 0.5 µg/ml)

Western Blotting (0.25-0.5 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10809 NCBI: NM_002156 NP_002147.2