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Catalog Number Size Price
LS-C312957-100 100 µg (0.5 mg/ml) $470 
HSPA2 Antibody - Western blot analysis of HSPA2 using anti-HSPA2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen Tissue Lysate, Lane 2: mouse testis tissue Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70KD. The expected band size for HSPA2 is at 70KD.
HSPA2 Antibody - WB of HSPA2 antibody. All lanes: Anti-HSPA2 at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 70KD. Observed bind size: 70KD.
HSPA2 Antibody - Western blot analysis of HSPA2 using anti-HSPA2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen Tissue Lysate, Lane 2: mouse testis tissue Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70KD. The expected band size for HSPA2 is at 70KD.
HSPA2 Antibody - WB of HSPA2 antibody. All lanes: Anti-HSPA2 at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 70KD. Observed bind size: 70KD.
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Polyclonal Rabbit anti‑Human HSPA2 Antibody (aa580‑599, WB) LS‑C312957

Polyclonal Rabbit anti‑Human HSPA2 Antibody (aa580‑599, WB) LS‑C312957

Note: This antibody replaces LS-C388618
Antibody:
HSPA2 Rabbit anti-Human Polyclonal (aa580-599) Antibody
Application:
WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Goat, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$470
LS-C312957-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPA2 Rabbit anti-Human Polyclonal (aa580-599) Antibody
Application:
WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Goat, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
HSPA2 antibody LS-C312957 is an unconjugated rabbit polyclonal antibody to HSPA2 (aa580-599) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
Target
Human HSPA2
Synonyms
HSPA2 | Heat shock 70kD protein 2 | HSP70-2 | HSP70-3 | Heat shock 70 kDa protein 2 | Heat shock 70kDa protein 2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Goat, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human HSPA2(580-599 aa VINWLDRNQMAEKDEYEHKQ), identical to the related mouse and rat sequences.
Epitope
aa580-599
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA2
P54652 NM_021979 NP_068814.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/9/2023