Mouse HMG1 / HMGB1

Hamster-Armenian

Mouse, Human, Rat (tested or 100% immunogen sequence identity)

IgG Monoclonal

Unconjugated

0.2 Micron Filter

Unmodified

• Immunofluorescence
• Western blot
• Flow Cytometry

FC: For staining cells were permeabilized with buffer containing 0.5% saponin. The cells were fixed in 0.01% formaldehyde before staining. Hamster IgG isotype control is used as a negative control. IF: NIH-3T3 cells were fixed with 2% PFA (15 min). After fixation, cells were washed with PBS and incubated for 5 min at 4ºC with permeabilization buffer . The cells were blocked with 1% BSA/10% goat serum in PBS for 10 min and were incubated with HMGB1 mAb 5H6 (10 ug/ml) overnight at 4ºC. The secondary antibody was biotinylated goat anti-hamster IgG at a 1:500 dilution for 1 hour. Finally, streptavidin was used at a 1:500 dilution for 30 min. W: A band is expected at ~30 kDa using reducing conditions. For non-reducing conditions a band is expected at both 27 kDa and 30 kDa.

Store at 4°C for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09429 NCBI: NM_002128 NP_002119.1