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Catalog Number Size Price
LS-C355110-100 100 µg (1 mg/ml) $504 
Histone H3 Antibody - Immunofluorescence analysis of Histone H3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Histone H3 (865R2) Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Histone H3 Antibody - WB using Histone H3 Antibody (865R2)
Histone H3 Antibody - Immunofluorescence analysis of Histone H3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Histone H3 (865R2) Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Histone H3 Antibody - WB using Histone H3 Antibody (865R2)
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Monoclonal Mouse anti‑Human Histone H3 Antibody (clone 865R2, IF, WB) LS‑C355110

Monoclonal Mouse anti‑Human Histone H3 Antibody (clone 865R2, IF, WB) LS‑C355110

Antibody:
Histone H3 Mouse anti-Human Monoclonal (865R2) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$504
LS-C355110-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Histone H3 Mouse anti-Human Monoclonal (865R2) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Histone H3 antibody LS-C355110 is an unconjugated mouse monoclonal antibody to Histone H3 from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human Histone H3
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgM,k Monoclonal
Clone
865R2
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Recombinant human histone H3 expressed in E. coli.
Applications
  • Immunofluorescence
  • Western blot
Presentation
PBS, pH 7.2, 0.1% Sodium Azide, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024