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Catalog Number Size Price
LS-C674395-50 50 µl $298 
LS-C674395-100 100 µl $370 
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HIST1H4I Antibody - Immunofluorescence staining of Hela cells with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HIST1H4I Antibody - Chromatin Immunoprecipitation Hela (4*10E6) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (HIST1H4A (Ab-20) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HIST1H4I Antibody - Immunofluorescence staining of Hela cells with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HIST1H4I Antibody - Chromatin Immunoprecipitation Hela (4*10E6) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (HIST1H4A (Ab-20) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
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Polyclonal Rabbit anti‑Human HIST1H4I Antibody (IHC, IF) LS‑C674395

Polyclonal Rabbit anti‑Human HIST1H4I Antibody (IHC, IF) LS‑C674395

Antibody:
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$298
LS-C674395-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST1H4I antibody LS-C674395 is an unconjugated rabbit polyclonal antibody to human HIST1H4I. Validated for ChrIP, ELISA, IF and IHC.
Target
Human HIST1H4I
Synonyms
HIST1H4I | HIST2H4 | Histone 1, H4i | Histone cluster 1, H4i | Histone family member | Histone H4 | Histone 4 family, member M
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Peptide sequence around site of Tri-methyl-Lys(20) derived from human Histone H4
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • ELISA
  • Chromatin Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST1H4I
P62805 NM_003495 NP_003486.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/15/2024