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HIST1H4I

histone Cluster 1, H4i

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33.

Gene Name: histone Cluster 1, H4i
Synonyms: HIST1H4I, HIST2H4, Histone 1, H4i, Histone cluster 1, H4i, Histone family member, Histone H4, Histone 4 family, member M
Target Sequences: NM_003495 NP_003486.1 P62805

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Products
Antibodies (122)
Primary (122)
HIST1H4I (122)
Human (122)
IHC (63)
IHC-P (59)
WB (58)
Flo (2)
ChrIP (25)
ELISA (118)
ICC (10)
IF (70)
IP (2)
rabbit (122)
IgG (122)
monoclonal mc (5)
polyclonal pc (117)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
Unconjugated (119)
aa50-C-term (2)
aa1-50 (1)
No (122)
HIST1H4I Antibody - Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Monoclonal Antibody
Human
ELISA, Flo, ICC, IF, WB
Unconjugated
50 µl/$316; 100 µl/$386
HIST1H4I Antibody - Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Monoclonal Antibody
Human
ELISA, ICC, IF, WB
Unconjugated
50 µl/$316; 100 µl/$386
HIST1H4I Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Monoclonal Antibody
Human
ELISA, Flo, IHC, IHC-P
Unconjugated
50 µl/$316; 100 µl/$386
HIST1H4I Antibody - Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Monoclonal Antibody
Human
ELISA, ICC, WB
Unconjugated
50 µl/$316; 100 µl/$386
HIST1H4I Antibody - Detection of Human Histone H4 by Immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti-Histone H4 used at a dilution of 1:200 (1 Detection: DAB.
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HIST1H4I Rabbit anti-Human Polyclonal (aa50-C-term) Antibody
Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
HIST1H4I Antibody - Immunofluorescence staining of Hela cells diluted at 1:50,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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HIST1H4I Rabbit anti-Human Monoclonal Antibody
Human
ELISA, IF
Unconjugated
50 µl/$316; 100 µl/$386
HIST1H4I Antibody - Immunocytochemistry analysis of Hela cells at a dilution of 1:100
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, ICC, IF, IHC, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image of paraffin-embedded human colon cancer at a dilution of 1:100
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image of paraffin-embedded human cervical cancer at a dilution of 1:100
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image of paraffin-embedded human cervical cancer at a dilution of 1:100
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry Dilution at 1:3 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry Dilution at 1:10 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, IF, IHC, IHC-P
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunocytochemistry analysis diluted at 1:10 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, ICC, IF, IHC
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry Dilution at 1:10 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry Dilution at 1:5 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image of paraffin-embedded human colon cancer at a dilution of 1:100
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:50 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:10 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry image at a dilution of 1:10 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunocytochemistry analysis diluted at 1:30 and staining in hela cells(treated with 30mM sodium butyrate for 4h)performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, ICC, IHC, WB
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry Dilution at 1:25 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, IF, IHC, IHC-P
Unconjugated
50 µl/$298; 100 µl/$370
HIST1H4I Antibody - Immunohistochemistry Dilution at 1:10 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Select
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50 µl/$298; 100 µl/$370
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).