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Catalog Number Size Price
LS-C746951-20 20 µl $275 
LS-C746951-100 100 µl $389 
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded human colon using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded human liver injury using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded mouse kidney using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded mouse liver using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded rat heart using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded human stomach using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded rat brain using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded mouse brain using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunofluorescence analysis of U2OS cells using H2AFX antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
H2AFX / H2AX Antibody - Western blot analysis of extracts of various cell lines, using H2AFX antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded human colon using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded human liver injury using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded mouse kidney using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded mouse liver using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded rat heart using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded human stomach using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded rat brain using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded mouse brain using H2AFX antibody at dilution of 1:100 (40x lens).
H2AFX / H2AX Antibody - Immunofluorescence analysis of U2OS cells using H2AFX antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
H2AFX / H2AX Antibody - Western blot analysis of extracts of various cell lines, using H2AFX antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (IHC, IF, WB) LS‑C746951

Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (IHC, IF, WB) LS‑C746951

Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C746951-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
H2AX antibody LS-C746951 is an unconjugated rabbit polyclonal antibody to H2AX (H2AFX) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human H2AFX / H2AX
Synonyms
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50 to the C-Terminus of human H2AFX (NP_002096.1). VYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQEY
Applications
  • IHC (1:100 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 15kDa, while the observed MW by Western blot was 16kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About H2AFX / H2AX
P16104 NM_002105 NP_002096.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024