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Catalog Number Size Price
LS-C41114-25 25 µg (0.5 mg/ml) $272 
LS-C41114-100 100 µg (0.5 mg/ml) $379 
H2AFX / H2AX Antibody - Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
H2AFX / H2AX Antibody - Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-H2A. X (Ser 139) antibody. Proteins were visualized using a goat anti-mouse secondary a.
H2AFX / H2AX Antibody - Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
H2AFX / H2AX Antibody - Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-H2A. X (Ser 139) antibody. Proteins were visualized using a goat anti-mouse secondary a.
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Monoclonal Mouse anti‑Human H2AFX / H2AX Antibody (clone 2F3, phospho‑Ser139, IF, WB) LS‑C41114

Monoclonal Mouse anti‑Human H2AFX / H2AX Antibody (clone 2F3, phospho‑Ser139, IF, WB) LS‑C41114

Note: This antibody replaces LS-C24262
Antibody:
H2AFX / H2AX Mouse anti-Human Monoclonal (pSer139) (2F3) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$272
LS-C41114-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
H2AFX / H2AX Mouse anti-Human Monoclonal (pSer139) (2F3) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
H2AX antibody LS-C41114 is an unconjugated mouse monoclonal antibody to human H2AX (H2AFX) (pSer139). Validated for ICC, IF and WB. Cited in 5 publications.
Target
Human H2AFX / H2AX
Synonyms
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
2F3
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human H2AFX / H2AX.
Epitope
pSer139
Specificity
Modified peptide (KATQAS*QEY)
Applications
  • ICC
  • Immunofluorescence
  • Western blot
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Storage
Store at 4°C. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About H2AFX / H2AX
P16104 NM_002105 NP_002096.1

LSBio Ratings

H2AFX / H2AX Antibody (phospho-Ser139, clone 2F3) for ICC, IF/Immunofluorescence, WB/Western LS-C41114 has an LSBio Rating of
Publications (4.4)

Publications (5)

Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Xiao R, Sun Y, Ding JH, Lin S, Rose DW, Rosenfeld MG, Fu XD, Li X. Molecular and cellular biology. 2007 27:5393-402.

Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Rossi DJ, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman IL. Nature. 2007 447:725-9.

DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. Mochizuki K, Novatchkova M, Loidl J. Journal of cell science. 2008 121:2148-58.

Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Akbay EA, Contreras CM, Perera SA, Sullivan JP, Broaddus RR, Schorge JO, Ashfaq R, Saboorian H, Wong KK, Castrillon DH. The American journal of pathology. 2008 173:536-44.

Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Loidl J, Mochizuki K. Molecular biology of the cell. 2009 20:2428-37.

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/11/2022