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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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H2AFX / H2AX

H2A histone family, member X

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Gene Name: H2A histone family, member X
Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone
Target Sequences: NM_002105 NP_002096.1 P16104

Publications (12)

1
Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Xiao R, Sun Y, Ding JH, Lin S, Rose DW, Rosenfeld MG, Fu XD, Li X. Molecular and cellular biology. 2007 27:5393-402. [PubMed:17526736] [PMC:PMC1952092] Related Antibodies: LS-C41114.
2
Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Rossi DJ, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman IL. Nature. 2007 447:725-9. [PubMed:17554309] Related Antibodies: LS-C41114.
3
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. Mochizuki K, Novatchkova M, Loidl J. Journal of cell science. 2008 121:2148-58. [PubMed:18522989] [PMC:PMC3184542] Related Antibodies: LS-C41114.
4
Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Akbay EA, Contreras CM, Perera SA, Sullivan JP, Broaddus RR, Schorge JO, Ashfaq R, Saboorian H, Wong KK, Castrillon DH. The American journal of pathology. 2008 173:536-44. [PubMed:18599611] [PMC:PMC2475790] Related Antibodies: LS-C41114.
5
Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Loidl J, Mochizuki K. Molecular biology of the cell. 2009 20:2428-37. [PubMed:19297526] [PMC:PMC2675622] Related Antibodies: LS-C41114.
6
gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?. Beels L, Bacher K, De Wolf D, Werbrouck J, Thierens H. Circulation. 2009 120:1903-9. [PubMed:19858412]
7
Tumor regression and curability of preclinical neuroblastoma models by PEGylated SN38 (EZN-2208), a novel topoisomerase I inhibitor. Pastorino F, Loi M, Sapra P, Becherini P, Cilli M, Emionite L, Ribatti D, Greenberger LM, Horak ID, Ponzoni M. Clinical cancer research : an official journal of the American Association for Cancer Research. 2010 16:4809-21. (IHC-Fr; Mouse) [PubMed:20702613]
8
Reduced NR4A gene dosage leads to mixed myelodysplastic/myeloproliferative neoplasms in mice. Ramirez-Herrick AM, Mullican SE, Sheehan AM, Conneely OM. Blood. 2011 117:2681-90. [PubMed:21205929] [PMC:PMC3062356]
9
The SET2-RPB1 interaction domain of human RECQ5 is important for transcription-associated genome stability. Li M, Xu X, Liu Y. Molecular and cellular biology. 2011 31:2090-9. [PubMed:21402780] [PMC:PMC3133350]
10
The nucleolus directly regulates p53 export and degradation. Boyd MT, Vlatkovic N, Rubbi CP. The Journal of cell biology. 2011 194:689-703. [PubMed:21893597] [PMC:PMC3171122]
11
Variant repeats are interspersed throughout the telomeres and recruit nuclear receptors in ALT cells. Conomos D, Stutz MD, Hills M, Neumann AA, Bryan TM, Reddel RR, Pickett HA. The Journal of cell biology. 2012 199:893-906. (ICC; Human) [PubMed:23229897] [PMC:PMC3518223]
12
Ionizing Radiation-Induced -H2AX Activity in Whole Blood Culture and the Risk of Lung Cancer. He Y, Gong Y, Lin J, Chang DW, Gu J, Roth JA, Wu X. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2013 22:443-51. [PubMed:23300022] [PMC:PMC3601549]
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Viewing 1-25 of 130 items
H2AFX / H2AX Antibody - Anti-H2AX antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50µl   1mg/ml   $425.00
H2AFX / H2AX Antibody - Anti-H2AX antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Human
IF, IHC, IHC-P, WB
Unconjugated
50µg   1mg/ml   $485.00
H2AFX / H2AX Antibody - Anti-H2AX antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Human H2AFX / H2AX
Rabbit, Rat, Bat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl   $485.00
H2AFX / H2AX Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Human
ELISA, IHC, IHC-P
Unconjugated
50µg   $425.00
Select
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Human
ELISA, WB
FITC Conjugated
50µg   0.25mg/ml   $285.00; 100µg   $385.00
H2AFX / H2AX Antibody - Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
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(5) Publication(s)
Mouse Monoclonal [clone 2F3] (IgG2b) to Human H2AFX / H2AX
Human
ICC, IF, WB
Unconjugated
25µg   0.5mg/ml   $225.00; 100µg   0.5mg/ml   $325.00
H2AFX / H2AX Antibody - Detection of Human H2AX by Immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
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Goat Polyclonal (IgG) to Human H2AFX / H2AX
Mouse, Human
IHC, WB
Unconjugated
100µl   1mg/ml   $395.00
Select
Rabbit Monoclonal [clone 20E3] (IgG) to Human H2AFX / H2AX
Mouse, Rat, Monkey
Flo, IF, IHC, WB
Unconjugated
100µl   $575.00
H2AFX / H2AX Antibody - Detection of Human gamma-H2AX by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-gamma-H2AX ( Lot12) used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
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Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Mouse, Human
ICC, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $415.00
H2AFX / H2AX Antibody - Immunofluorescence of monoclonal antibody to H2AFX on HeLa cell . [antibody concentration 10 ug/ml]
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Mouse Monoclonal [clone 3F4] (IgG2a,k) to Human H2AFX / H2AX
Human
ELISA, IF
Unconjugated
100µg   $365.00
H2AFX / H2AX Antibody - IHC of p-Histone H2A.X (S139) pAb in paraffin-embedded human brain tissue.
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Rabbit Polyclonal to Human H2AFX / H2AX
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl   $345.00
H2AFX / H2AX Antibody - IHC testing of FFPE mouse liver with phospho-Histone H2AX antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Rabbit Monoclonal [clone AbH41] (IgG) to Human H2AFX / H2AX
Mouse, Human
IF, IHC-P, WB
Unconjugated
100µg   $435.00
Select
Rabbit Monoclonal (IgG) to Human H2AFX / H2AX
Human
ELISA, ICC, WB
Unconjugated
25µg   $335.00
H2AFX / H2AX Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Rabbit Polyclonal (IgG) to Mouse H2AFX / H2AX
Mouse, Rat
IHC-P, WB
Unconjugated
50µl   $235.00; 100µl   $265.00; 200µl   $335.00
H2AFX / H2AX Antibody - Western Blot Positive WB detected in:293 whole cell lysate All Lanes:Phospho-Histone H2AX (S139) antibody at 0.23µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
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Rabbit Monoclonal (IgG) to Human H2AFX / H2AX
Human
ELISA, WB
Unconjugated
50µl   $315.00; 100µl   $425.00
H2AFX / H2AX Antibody - Immunohistochemistry image at a dilution of 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µl   $295.00; 100µl   $395.00
H2AFX / H2AX Antibody - Immunohistochemical analysis of Histone H2A.X staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Rabbit Polyclonal to Human H2AFX / H2AX
Rabbit, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
H2AFX / H2AX Antibody - Immunohistochemical analysis of Histone H2A.X staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human H2AFX / H2AX
Mouse, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
30µl   1mg/ml   $205.00; 100µl   1mg/ml   $245.00; 200µl   1mg/ml   $305.00
Select
Rabbit Monoclonal [clone 20E3] (IgG) to Human H2AFX / H2AX
Mouse, Rat, Human, Monkey
Flo
PE Conjugated
100µl   $605.00
Select
Rabbit Polyclonal to Human H2AFX / H2AX
Human
ELISA, IF, IHC, WB
Unconjugated
100µg   100µl   1mg/ml   $315.00
H2AFX / H2AX Antibody - Western blot of Histone H2A.X antibody
Select
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
Select
Mouse Monoclonal (IgG1) to Human H2AFX / H2AX
Rabbit, Dog, Bovine, Human, Monkey
ICC, IF, IP, WB
Unconjugated
100µg   $530.00
H2AFX / H2AX Antibody - Western blot of Histone H2A.X antibody
Select
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded human stomach using H2AFX antibody at dilution of 1:100 (40x lens).
Select
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl   $235.00; 100µl   $295.00; 200µl   $385.00
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded rat liver using H2AFX antibody at dilution of 1:100 (40x lens).
Select
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl   $235.00

Viewing 1-25 of 130 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).