Glutamate Dehydrogenase antibody LS-B2525 is an unconjugated rabbit polyclonal antibody to Glutamate Dehydrogenase (GLUD1) from human and bovine. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Bovine GLUD1/Glutamate Dehydrogenase
(tested or 100% immunogen sequence identity)
Ion exchange chromatography
- IHC - Paraffin (5 µg/ml)
- Western blot
- ELISA (1:6000 - 1:24000)
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Bovine liver glutamate dehydrogenase.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum as well as purified and partially purified bovine liver glutamate dehydrogenase.
Immunohistochemistry: LS-B2525 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2525 was determined to be 5 ug/ml.
PBS, pH 7.2, 0.01% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About GLUD1/Glutamate Dehydrogenase