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IHCPlus™ GLUD1/Glutamate Dehydrogenase Antibody aa93‑107 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B8318

Catalog Number Size Price
LS-B8318-50 50 µg (0.5 mg/ml) $425 
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-Glutamate Dehydrogenase Antibody (0.01?/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35? protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-Glutamate Dehydrogenase Antibody (0.03?/ml) staining of Human (A) and Mouse (B) Brain lysate (35? protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GLUD1 antibody (0.3 ug/ml) staining of Mouse Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-Glutamate Dehydrogenase Antibody (0.01?/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35? protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-Glutamate Dehydrogenase Antibody (0.03?/ml) staining of Human (A) and Mouse (B) Brain lysate (35? protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GLUD1 antibody (0.3 ug/ml) staining of Mouse Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ GLUD1/Glutamate Dehydrogenase Antibody aa93‑107 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B8318

Note: This antibody replaces LS-C112934
Antibody:
Goat Polyclonal to Human GLUD1/Glutamate Dehydrogenase
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Horse, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8318-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human GLUD1/Glutamate Dehydrogenase
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Horse, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Glutamate Dehydrogenase antibody LS-B8318 is an unconjugated goat polyclonal antibody to Glutamate Dehydrogenase (GLUD1) from human, mouse, horse and other species. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GLUD1/Glutamate Dehydrogenase
Host
Goat
Reactivity
Human, Monkey, Mouse, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-ESEEQKRNRVRGILR, from the internal region of the protein sequence according to NP_005262.1NP_036216.2.
Blocking Peptide
LS-E27293 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8318. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa93-107
Specificity
Human GLUD1. This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kDa band observed in Human Liver and in Mouse Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1 and NP_036216.2 and of 61.3 according to Mouse NP_032159.1. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GLUD1/Glutamate Dehydrogenase
P00367 NM_005271 NP_005262.1

Validation

Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IHC Validated
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Requested From: United States
Date Requested: 8/19/2019
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