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Catalog Number Size Price
LS-C745582-25 25 µl (0.95 mg/ml) $275 
GDF15 Antibody - Western blot using the affinity purified anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Mouse GDF15 Antibody (C‑Terminus, WB) LS‑C745582

Polyclonal Rabbit anti‑Mouse GDF15 Antibody (C‑Terminus, WB) LS‑C745582

Antibody:
GDF15 Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745582-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
GDF15 Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
GDF15 antibody LS-C745582 is an unconjugated rabbit polyclonal antibody to GDF15 (C-Terminus) from mouse. It is reactive with human and mouse. Validated for ELISA and WB.
Target
Mouse GDF15
Synonyms
GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Predicted
Bovine, Dog, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared by repeated immunizations with a peptide corresponding to an amino acid sequence near the C-terminal of mouse NAG-1 protein.
Epitope
C-Terminus
Specificity
This antibody reacts with endogenous NAG-1 protein from mouse and human tissues. A BLAST analysis suggests reactivity with NAG-1 from rat based on 100% homology. Partial reactivity is expected against swine, bovine and dog based on 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:100000 - 1:120000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GDF15
Q99988 NM_004864 NP_004855.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021