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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B8327-50 50 µg (1 mg/ml) $425 
GDF15 Antibody - Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
GDF15 Antibody - Western Blot of Rat Anti-Nag 1 (C terminal specific). Lane 1: Protein Standard Opal Pre-stained  Lane 2: MBP-NAG-1 fusion protein 1. Lane 3: MBP-NAG-1 fusion protein 2. Lane 4: MBP. Load 40ng. Primary Antibody: Rat anti-Nag 1 (C terminal specific) at 1 µg/mL for overnight at 4°C. Secondary Antibody: Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed at 1:40,000 dilution at RT for 30 minutes.
GDF15 Antibody - Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
GDF15 Antibody - Western Blot of Rat Anti-Nag 1 (C terminal specific). Lane 1: Protein Standard Opal Pre-stained  Lane 2: MBP-NAG-1 fusion protein 1. Lane 3: MBP-NAG-1 fusion protein 2. Lane 4: MBP. Load 40ng. Primary Antibody: Rat anti-Nag 1 (C terminal specific) at 1 µg/mL for overnight at 4°C. Secondary Antibody: Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed at 1:40,000 dilution at RT for 30 minutes.
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IHCPlus™ Monoclonal GDF15 Antibody (clone 6D12.H10.E4, C‑Terminus, IHC, WB) LS‑B8327

IHCPlus™ Monoclonal GDF15 Antibody (clone 6D12.H10.E4, C‑Terminus, IHC, WB) LS‑B8327

Note: This antibody replaces LS-C153803
Antibody:
Rat Monoclonal to Human GDF15
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8327-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rat Monoclonal to Human GDF15
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
GDF15 antibody LS-B8327 is an unconjugated rat monoclonal antibody to GDF15 (C-Terminus) from human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GDF15
Synonyms
GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
Host
Rat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG2a,l Monoclonal
Clone
6D12.H10.E4
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
  • ELISA (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein.  Cross reactivity to MBP is negative.
Epitope
C-Terminus
Specificity
This antibody reacts with the C-terminus of endogenous NAG-1 protein from mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Usage
This Protein G purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately 13 and 26 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Expiration date is one year from opening.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GDF15
Q99988 NM_004864 NP_004855.2

Validation

GDF15 Antibody - Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

LSBio Ratings

IHC-plus™ GDF15 Antibody (C-Terminus, clone 6D12.H10.E4) for IHC, WB/Western, ELISA LS-B8327 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 3/30/2020