FOXP3 antibody LS-C391856 is an unconjugated mouse monoclonal antibody to FOXP3 from human and mouse. Validated for Flow, IF and IHC.
(tested or 100% immunogen sequence identity)
Protein G purified
Also available Unmodified
- IHC - Paraffin (1:1)
- Flow Cytometry
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
FOXP3 antibody was raised against n-terminal recombinant protein fragment.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation. Titering of the FOXP3 antibody may be required for optimal performance. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
PBS, 0.01% BSA, 0.05% sodium azide
Store at 2°C to 8°C.
For research use only.