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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXP3 Antibody (C-Terminus) LS-C20231

The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and analyzed by Western blot with anti-Foxp3 antiserum (1:2000 dilution).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of immunoprecipitated FOXP3 from HA-tagged FOXP3 transfected Jurkat T antigen cells. Immunoprecipitated .  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfectants from A were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of positively stained cells are shown.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and analyzed by Western blot with anti-Foxp3 antiserum (1:2000 dilution).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of immunoprecipitated FOXP3 from HA-tagged FOXP3 transfected Jurkat T antigen cells. Immunoprecipitated .  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfectants from A were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of positively stained cells are shown.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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FOXP3 Antibody (C-Terminus) LS-C20231

Antibody:
Rabbit Polyclonal to Mouse FOXP3
Application:
ICC, IF, WB, Flo
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$335 
LS-C20231-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse FOXP3
Application:
ICC, IF, WB, Flo
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
FOXP3 antibody LS-C20231 is an unconjugated rabbit polyclonal antibody to FOXP3 from human and mouse. Validated for Flow, ICC, IF and WB.

Target
Mouse FOXP3
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence
  • Western blot (1:2000)
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Immunogen
FOXP3 antibody was raised against a fusion protein, GST-mouse Foxp3-Flag. Mouse Foxp3 Gly43 to His100 peptide and Flag Tag were fused at C-terminal of GST protein
Epitope
C-Terminus
Specificity
FOXP3.
Presentation
PBS, 0.05% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOXP3
Q9BZS1 NM_014009 NP_054728.2

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Featured Products

Product
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
Product
Species: Human
Applications: IHC, IHC - Paraffin
Reactivity: Mouse
Range: 15.6-1000 pg/ml
Reactivity: Human
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 6/16/2019
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