FOXP3 antibody LS-C66364 is an unconjugated rabbit polyclonal antibody to mouse FOXP3. Validated for Flow, ICC and WB.
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot
- Flow Cytometry
FOXP3 antibody was raised against a fusion protein, GST-mouse Foxp3-Flag. Mouse Foxp3 Gly43 to His100 peptide and Flag Tag were fused at C-terminal of GST protein.
Species cross-reactivity: This antibody has shown reactivity against mouse protein (CD4+CD25+ T cells). There appears to be no reactivity against human Jurkat and mouse CD4+CD25- T cell lysates. Other species and samples have not been tested.
Suitable for use in FACS/Flow Cytometry, Western Blot and Immunocytochemistry. Recommended Dilutions Western Blot: Band is seen at ~48kD in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. Positive Control: Mouse CD4+CD25+ T cells
Tris-Glycine, NaCl, 0.05% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.