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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXP3 Antibody (N‑Terminus, clone 3G3, Azide‑free) LS‑C368324
FOXP3 antibody LS-C368324 is an unconjugated mouse monoclonal antibody to FOXP3 from human and mouse. Validated for Flow and WB.
Catalog
Size
Price
LS-C368324-100
100 µg (0.5 mg/ml)
$365
Description
FOXP3 antibody LS-C368324 is an unconjugated mouse monoclonal antibody to FOXP3 from human and mouse. Validated for Flow and WB.
Target
Mouse FOXP3
Host
Mouse
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [3G3]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free
Applications
  • Western blot (2 µg/ml)
  • Flow Cytometry
Immunogen
FOXP3 antibody was raised against full-length His-tagged murine FOXP3. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849).
Epitope
N-Terminus
Specificity
Mouse FOXP3
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, 0.02% gelatin
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FOXP3
Q9BZS1 NM_014009 NP_054728.2

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The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: ICC, Immunofluorescence, Western blot, Flow Cytometry
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Species: Mouse
Applications: IHC, IHC - Frozen, Flow Cytometry

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Images

Western blot

Western Blot: FOXP3 Antibody (3G3) [Azide Free] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Western Blot: FOXP3 Antibody (3G3) [Azide Free] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: FOXP3 Antibody (3G3) [Azide Free] - Detection of FOXP3 in approximately 2x10^6 mouse lymph node cells using 100 ng (in 50 ul total volume) of FOXP3 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow Cytometry: FOXP3 Antibody (3G3) [Azide Free] - Detection of FOXP3 in approximately 2x10^6 mouse lymph node cells using 100 ng (in 50 ul total volume) of FOXP3 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western Blot: FOXP3 Antibody (3G3) [Azide Free] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Western Blot: FOXP3 Antibody (3G3) [Azide Free] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: FOXP3 Antibody (3G3) [Azide Free] - Detection of FOXP3 in approximately 2x10^6 mouse lymph node cells using 100 ng (in 50 ul total volume) of FOXP3 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow Cytometry: FOXP3 Antibody (3G3) [Azide Free] - Detection of FOXP3 in approximately 2x10^6 mouse lymph node cells using 100 ng (in 50 ul total volume) of FOXP3 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western Blot: FOXP3 Antibody (3G3) [Azide Free] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Western Blot: FOXP3 Antibody (3G3) [Azide Free] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: FOXP3 Antibody (3G3) [Azide Free] - Detection of FOXP3 in approximately 2x10^6 mouse lymph node cells using 100 ng (in 50 ul total volume) of FOXP3 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow Cytometry: FOXP3 Antibody (3G3) [Azide Free] - Detection of FOXP3 in approximately 2x10^6 mouse lymph node cells using 100 ng (in 50 ul total volume) of FOXP3 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western Blot: FOXP3 Antibody (3G3) [Azide Free] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Western Blot: FOXP3 Antibody (3G3) [Azide Free] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: FOXP3 Antibody (3G3) [Azide Free] - Detection of FOXP3 in approximately 2x10^6 mouse lymph node cells using 100 ng (in 50 ul total volume) of FOXP3 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow Cytometry: FOXP3 Antibody (3G3) [Azide Free] - Detection of FOXP3 in approximately 2x10^6 mouse lymph node cells using 100 ng (in 50 ul total volume) of FOXP3 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/18/2018
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