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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXP3 Antibody LS‑C355650
FOXP3 antibody LS-C355650 is an unconjugated rabbit polyclonal antibody to FOXP3 from human, monkey and orangutan. Validated for ChrIP, IF and WB.
Catalog
Size
Price
LS-C355650-100
100 µg
$385
Description
FOXP3 antibody LS-C355650 is an unconjugated rabbit polyclonal antibody to FOXP3 from human, monkey and orangutan. Validated for ChrIP, IF and WB.
Target
Human FOXP3
Host
Rabbit
Reactivity
Human, Orangutan, Monkey (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:500)
  • Chromatin Immunoprecipitation
Immunogen
FOXP3 antibody was raised against synthetic peptide corresponding to aa406-420 (WTVDELEFRKKRSQR) of human FOXP3. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey (100%); Galago, Mouse, Rat, Sheep, Dog, Cat, Bovine, Horse, Pig (93%).
Specificity
The antibody detects FOXP3 from human samples. It has been successfully used in Western blot and ChIP procedures. By Western blot, It detects a ~50 kD band representing FOXP3 from HeLa cells.
Presentation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FOXP3
Q9BZS1 NM_014009 NP_054728.2

Popular FOXP3 Products

FOXP3 antibody ARP32743_T100-NP_054728-FOXP3 (forkhead box P3) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bovine, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
IHC of FOXP3 on FFPE Tonsil tissue Intended Use For In Vitro Diagnostic Use.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
Antibody
Species: Mouse
Applications: ICC, Western blot, Flow Cytometry
The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: ICC, Immunofluorescence, Western blot, Flow Cytometry
Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Flow Cytometry

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Images

Immunofluorescence

Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Western blot

WB using FOXP3 Antibody
WB using FOXP3 Antibody

Chromatin Immunoprecipitation

ChIP using FOXP3 Antibody
ChIP using FOXP3 Antibody

Immunofluorescence

Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Western blot

WB using FOXP3 Antibody
WB using FOXP3 Antibody

Chromatin Immunoprecipitation

ChIP using FOXP3 Antibody
ChIP using FOXP3 Antibody

Immunofluorescence

Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Western blot

WB using FOXP3 Antibody
WB using FOXP3 Antibody

Chromatin Immunoprecipitation

ChIP using FOXP3 Antibody
ChIP using FOXP3 Antibody

Immunofluorescence

Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Western blot

WB using FOXP3 Antibody
WB using FOXP3 Antibody

Chromatin Immunoprecipitation

ChIP using FOXP3 Antibody
ChIP using FOXP3 Antibody

Requested From: United States
Date Requested: 12/10/2018
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