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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXP3 Antibody LS-C355650

Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
WB using FOXP3 Antibody
ChIP using FOXP3 Antibody
Immunofluorescence analysis of FOXP3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXP3 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
WB using FOXP3 Antibody
ChIP using FOXP3 Antibody
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FOXP3 Antibody LS-C355650

Antibody:
Rabbit Polyclonal to Human FOXP3
Application:
IF, WB, ChrIP
Reactivity:
Human, Orangutan, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$385 
LS-C355650-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human FOXP3
Application:
IF, WB, ChrIP
Reactivity:
Human, Orangutan, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
FOXP3 antibody LS-C355650 is an unconjugated rabbit polyclonal antibody to FOXP3 from human, monkey and orangutan. Validated for ChrIP, IF and WB.

Target
Human FOXP3
Host
Rabbit
Reactivity
Human, Orangutan, Monkey (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:500)
  • Chromatin Immunoprecipitation
Immunogen
FOXP3 antibody was raised against synthetic peptide corresponding to aa406-420 (WTVDELEFRKKRSQR) of human FOXP3. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey (100%); Galago, Mouse, Rat, Sheep, Dog, Cat, Bovine, Horse, Pig (93%).
Specificity
The antibody detects FOXP3 from human samples. It has been successfully used in Western blot and ChIP procedures. By Western blot, It detects a ~50 kD band representing FOXP3 from HeLa cells.
Presentation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOXP3
Q9BZS1 NM_014009 NP_054728.2

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Featured Products

Reactivity: Rat
Range: 0.156-10 ng/ml
Reactivity: Monkey
Range: 31.25-2000 pg/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Product
Species: Human, Bovine, Pig
Applications: ELISA

Requested From: United States
Date Requested: 6/24/2019
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