Products
Services
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C11577-0.1 0.1 ml $265 
LS-C11577-1 1 ml $1,425 
Antibody

Monoclonal Mouse anti‑Human ER Alpha / Estrogen Receptor Antibody (clone 6F11, Supernatant, IHC) LS‑C11577

Monoclonal Mouse anti‑Human ER Alpha / Estrogen Receptor Antibody (clone 6F11, Supernatant, IHC) LS‑C11577

Note: This antibody replaces LS-C43920
Antibody:
Mouse Monoclonal to Human ER Alpha / Estrogen Receptor
Application:
IHC-P, IHC-Fr
Reactivity:
Human
Format:
Unconjugated, Supernatant
Price
Catalog Number
$265 
LS-C11577-0.1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human ER Alpha / Estrogen Receptor
Application:
IHC-P, IHC-Fr
Reactivity:
Human
Format:
Unconjugated, Supernatant

Specifications

Description
Estrogen Receptor antibody LS-C11577 is an unconjugated mouse monoclonal antibody to human Estrogen Receptor (ER Alpha). Validated for IHC.
Target
Human ER Alpha / Estrogen Receptor
Synonyms
ESR1 | Era | ERalpha | Estrogen receptor | ER-alpha | Estrogen receptor 1 | NR3A1 | ER | ESR | ESRA | Estrogen receptor alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
6F11
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Supernatant
Applications
  • IHC - Paraffin (1:40 - 1:80)
  • IHC - Frozen (1:40 - 1:60)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human estrogen receptor (alpha form).
Specificity
Recognizes the human estrogen receptor alpha chain (ERalpha), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERalpha is a ~65kD steroid hormone receptor containing an N-terminal. ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an. ERalpha binds to DNA as a homodimer and can also form heterodimers with Estrogen receptor beta The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy. Has been used successfully for identification of ERalpha on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERalpha in breast cancer tissues.
Usage
Immunohistology: Zamboni's fixative recommended for best results. #This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Presentation
0.09% Sodium Azide
Reconstitution
1 ml Distilled Water.
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ER Alpha / Estrogen Receptor
P03372 NM_000125 NP_000116.2

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 7/9/2020