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Catalog Number Size Price
LS-C23274-50 50 µg $551 
LS-C23274-200 200 µg $859 
Antibody

Monoclonal Mouse anti‑Human ER Alpha / Estrogen Receptor Antibody (clone C0542, aa582‑595, IHC, WB) LS‑C23274

Monoclonal Mouse anti‑Human ER Alpha / Estrogen Receptor Antibody (clone C0542, aa582‑595, IHC, WB) LS‑C23274

Antibody:
ER Alpha / Estrogen Receptor Mouse anti-Human Monoclonal (aa582-595) (C0542) Antibody
Application:
IHC, ICC, WB, IP, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$551
LS-C23274-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ER Alpha / Estrogen Receptor Mouse anti-Human Monoclonal (aa582-595) (C0542) Antibody
Application:
IHC, ICC, WB, IP, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Estrogen Receptor antibody LS-C23274 is an unconjugated mouse monoclonal antibody to human Estrogen Receptor (ER Alpha) (aa582-595). Validated for GS, ICC, IHC, IP and WB.
Target
Human ER Alpha / Estrogen Receptor
Synonyms
ESR1 | Era | ERalpha | Estrogen receptor | ER-alpha | Estrogen receptor 1 | NR3A1 | ER | ESR | ESRA | Estrogen receptor alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
C0542
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A 14 residue C-Terminus synthetic peptide, (C)YYITGEAEGFPATV based on the human estrogen receptor (aa 582-595) alpha protein sequence with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%); Gorilla, Monkey (93%); Bat, Bovine, Horse (86%).
Epitope
aa582-595
Specificity
Recognizes the human estrogen receptor at ~65kD and ~55kD believed to be the major proteolytic fragment of the estrogen receptor alpha protein. The antibody also recognizes native estrogen receptor in both the inactive 8S oligomeric state, when activated to 4S and whether unbound or bound to ligand. Species cross-reactivity: mouse, rat and hamster.
Applications
  • IHC
  • ICC
  • Western blot
  • Immunoprecipitation
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot, Gel Mobility Shift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (ECL): 2 ug/ml. Gel Mobility Shift Assay: 20 ug/reaction. Immunoprecipitation: 20 ug/100 ul beads. We recommend that protein A Sepharose be used as immunoabsorbant with a bridging rabbit anti-mouse IgG to ensure efficient binding of the antibody.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ER Alpha / Estrogen Receptor
P03372 NM_000125 NP_000116.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/14/2022