Target
Mouse EIF4EBP1 / 4EBP1
Synonyms
EIF4EBP1 | 4E-BP1 | 4EBP1 | BP-1 | PHAS-I | EIF4E-binding protein 1
Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.
Specificity
Detects endogenous levels of 4E-BP1 only when phosphorylated at Thr70. Does not detect 4E-BP1 phosphorylated at other sites. Species cross-Reactivity: human, mouse, rat.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:50)
Usage
Suitable for use in Western Blot, and Immunoprecipitation. Western Blot: 1:1000 (Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking.). Immunoprecipitation: 1:50.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee