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Catalog Number Size Price
LS-B3748-50 50 µg (0.9 mg/ml) $460 
EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC‑plus™ Polyclonal Rabbit anti‑Human EIF4EBP1 / 4EBP1 Antibody (aa101‑118, IHC, WB) LS‑B3748

IHC‑plus™ Polyclonal Rabbit anti‑Human EIF4EBP1 / 4EBP1 Antibody (aa101‑118, IHC, WB) LS‑B3748

Note: This antibody replaces LS-C63173
Antibody:
EIF4EBP1 / 4EBP1 Rabbit anti-Human Polyclonal (aa101-118) Antibody
Application:
IHC, IHC-P, WB, GS
Reactivity:
Human, Mouse, Rat, Hamster, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3748-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
EIF4EBP1 / 4EBP1 Rabbit anti-Human Polyclonal (aa101-118) Antibody
Application:
IHC, IHC-P, WB, GS
Reactivity:
Human, Mouse, Rat, Hamster, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
4EBP1 antibody LS-B3748 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1) (aa101-118) from human. It is reactive with human, mouse, rat and other species. Validated for GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EIF4EBP1 / 4EBP1
Synonyms
EIF4EBP1 | 4E-BP1 | 4EBP1 | BP-1 | PHAS-I | EIF4E-binding protein 1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Dog, Goat, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (aa 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%); Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%); Panda (83%).
Epitope
aa101-118
Specificity
Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (4 µg/ml)
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3748 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3748 was determined to be 10 ug/ml.
Presentation
25 mM Sodium Borate, BBS, pH 8.4, 75 mM NaCl, 5 mM EDTA, 100 mM Boric acid
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF4EBP1 / 4EBP1
Q13541 NM_004095 NP_004086.1

Validation

EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ EIF4EBP1 / 4EBP1 Antibody (aa101-118) for IHC, WB/Western LS-B3748 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/8/2024