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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B3748-50 50 µg (0.9 mg/ml) $425 
EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHCPlus™ EIF4EBP1 / 4EBP1 Antibody aa101‑118 Polyclonal GS,IHC,WB LS‑B3748

IHCPlus™ EIF4EBP1 / 4EBP1 Antibody aa101‑118 Polyclonal GS,IHC,WB LS‑B3748

Note: This antibody replaces LS-C63173
Antibody:
Rabbit Polyclonal to Human EIF4EBP1 / 4EBP1
Application:
IHC, IHC-P, WB, GS
Reactivity:
Human, Mouse, Rat, Hamster, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3748-50
50 µg
0.9 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human EIF4EBP1 / 4EBP1
Application:
IHC, IHC-P, WB, GS
Reactivity:
Human, Mouse, Rat, Hamster, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
4EBP1 antibody LS-B3748 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1 ) from human, mouse, rat and other species. Validated for GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EIF4EBP1 / 4EBP1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Dog, Goat, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (4 µg/ml)
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%); Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%); Panda (83%).
Epitope
aa101-118
Specificity
Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
Usage
Immunohistochemistry: LS-B3748 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3748 was determined to be 10 ug/ml.
Presentation
25 mM Sodium Borate, BBS, pH 8.4, 75 mM NaCl, 5 mM EDTA, 100 mM Boric acid
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF4EBP1 / 4EBP1
Q13541 NM_004095 NP_004086.1

Validation

EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

EIF4EBP1 / 4EBP1 Antibody - Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 11/20/2019