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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF4EBP1 / 4EBP1

eukaryotic translation initiation factor 4E binding protein 1

EIF4EBP1 / 4EBP1 is one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Gene Name: eukaryotic translation initiation factor 4E binding protein 1
Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1
Target Sequences: NM_004095 NP_004086.1 Q13541

Filtered By: None
Target
EIF4EBP1 / 4EBP1 (157)
Reactivity
Human (131)
Mouse (79)
Rat (71)
Bat (4)
Bovine (12)
Chicken (4)
Dog (7)
Drosophila (1)
Guinea pig (4)
Hamster (5)
Horse (7)
Monkey (14)
Pig (9)
Rabbit (10)
Zebrafish (2)
Application
IHC (95)
IHC-P (28)
WB (128)
Flo (9)
DB (8)
ELISA (53)
GS (1)
ICC (6)
IF (12)
IP (3)
Peptide-ELISA (11)
Host
rabbit (137)
mouse (20)
Isotype
IgG (99)
IgG1 (7)
IgG1,k (9)
Clonality
monoclonal mc (23)
polyclonal pc (134)
Clone
11G12C11 (1)
1E2E7 (1)
2D1G11 (1)
319CT22.8.2 (7)
4B6G10 (2)
4F3-H2 (1)
9E12D9 (1)
Format
AP Conjugated (4)
APC (3)
APC Conjugated (6)
Azide-free (3)
Biotin Conjugated (11)
Cy3 Conjugated (3)
Cy7 Conjugated (3)
FITC Conjugated (12)
HRP Conjugated (10)
PE Conjugated (8)
Unconjugated (100)
Epitope
aa2-117 (17)
pThr36 (13)
C-Terminus (8)
Thr69 (8)
aa2-118 (8)
10-90 aa (7)
pSer65 (6)
aa21-70 (4)
pThr45 (4)
pThr69 (4)
pThr70 (3)
30-110 aa (2)
40-120 aa (2)
N-Terminus (2)
pSer64 (2)
pThr37 (2)
pThr37/Thr46 (2)
pThr46 (2)
Asn63 (1)
Gly39 (1)
Internal (1)
Leu58 (1)
Pro30 (1)
Thr37/Thr46 (1)
Thr46 (1)
aa101-118 (1)
aa13-62 (1)
aa2-51 (1)
aa30-79 (1)
aa31-61 (1)
aa35-84 (1)
aa36-85 (1)
aa4-53 (1)
pSer112 (1)
pThr37/46 (1)
Publications
No (157)
Showing 1-25 of 157 results

EIF4EBP1 / 4EBP1 Antibody (C-Terminus) IHC-plus™ LS-B564
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Human
ICC IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 ug/ml.

EIF4EBP1 / 4EBP1 Antibody (phospho-Ser65) LS-C122561
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Human, Monkey
ELISA IHC WB
Unconjugated
50µg 1mg/ml  $435.00

EIF4EBP1 / 4EBP1 Antibody (phospho-Thr37/Thr46) LS-C92051
Rabbit Monoclonal (IgG) to Mouse EIF4EBP1 / 4EBP1
Drosophila, Rat, Mouse, Human, Monkey
Flo IF IHC IHC-P WB
Unconjugated
100µl  $575.00

EIF4EBP1 / 4EBP1 Antibody (aa101-118) IHC-plus™ LS-B3748
Rabbit Polyclonal to Human EIF4EBP1 / 4EBP1
Rabbit, Mouse, Rat, Hamster, Human
GS IHC IHC-P WB
Unconjugated
50µg 0.9mg/ml  $425.00
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

EIF4EBP1 / 4EBP1 Antibody (aa35-84) IHC-plus™ LS-B12518
Rabbit Polyclonal (IgG) to Mouse EIF4EBP1 / 4EBP1
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
100µl 1mg/ml  $505.00
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

EIF4EBP1 / 4EBP1 Antibody (clone 4B6G10) LS-C82067
Mouse Monoclonal [clone 4B6G10] (IgG1) to Human EIF4EBP1 / 4EBP1
Human
ELISA IHC IHC-P WB
Unconjugated
100µg 1mg/ml  $545.00
IHC of paraffin-embedded human pancreas carcinoma showing cytoplasmic and membrane location using 4E-BP1 antibody with DAB staining.
Western blot of 4E-BP1 monoclonal antibody against truncated 4E-BP1 recombinant protein and A431 cell lysate.
IHC of paraffin-embedded human pancreas carcinoma showing cytoplasmic and membrane location using 4E-BP1 antibody with DAB staining.
Western blot of 4E-BP1 monoclonal antibody against truncated 4E-BP1 recombinant protein and A431 cell lysate.

EIF4EBP1 / 4EBP1 Antibody (Thr46) LS-C34757
Rabbit Monoclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Rat, Human, Monkey
ELISA Flo WB
Unconjugated
100µl  $575.00

EIF4EBP1 / 4EBP1 Antibody (phospho-Ser65) LS-C20243
Rabbit Polyclonal (IgG) to Mouse EIF4EBP1 / 4EBP1
Mouse, Rat, Human
ELISA IP WB
Unconjugated
100µl 0.3mg/ml  $575.00

EIF4EBP1 / 4EBP1 Antibody (phospho-Ser65, clone 1E2E7) LS-C682434
Mouse Monoclonal [clone 1E2E7] (IgG1) to Human EIF4EBP1 / 4EBP1
Human
ELISA Flo ICC IHC
Unconjugated
100µl  $365.00
Immunofluorescence analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Immunofluorescence analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.

EIF4EBP1 / 4EBP1 Antibody (Thr37/Thr46, Biotin) LS-C126495
Rabbit Monoclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Rat, Human, Monkey
Flo WB
Biotin Conjugated
100µl  $605.00

EIF4EBP1 / 4EBP1 Antibody (N-Terminus) LS-C356013
Rabbit Polyclonal to Human EIF4EBP1 / 4EBP1
Zebrafish, Pig, Mouse, Bovine, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of 4EBP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of 4EBP1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of 4EBP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of 4EBP1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

EIF4EBP1 / 4EBP1 Antibody (aa30-79) IHC-plus™ LS-B7558
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from Jurkat cells, treated with Insulin 0.01U/ml 15', using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from Jurkat cells, treated with Insulin 0.01U/ml 15', using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

EIF4EBP1 / 4EBP1 Antibody LS-C483126
Mouse Monoclonal to Human EIF4EBP1 / 4EBP1
Human
ELISA ICC IF WB
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

EIF4EBP1 / 4EBP1 Antibody (aa31-61) LS-C167710
Rabbit Polyclonal to Human EIF4EBP1 / 4EBP1
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $305.00
EIF4EBP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4EBP1 gene.
EIF4EBP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4EBP1 gene.

EIF4EBP1 / 4EBP1 Antibody (phospho-Ser65, clone 2D1G11) LS-C682433
Mouse Monoclonal [clone 2D1G11] (IgG1) to Human EIF4EBP1 / 4EBP1
Human
ELISA Flo IHC
Unconjugated
100µl  $365.00
Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.

EIF4EBP1 / 4EBP1 Antibody (C-Terminus) LS-C287384
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Rabbit, Mouse, Rat, Pig, Human
IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human 4EBP1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20000 (0.05 ug/ml).
Detection of 4EBP1 by Western Blot. Samples: Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody: Affinity purified rabbit anti-4EBP1 used at 0.25 ug/ml. Detection: Chemiluminescence.
Detection of Human 4EBP1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20000 (0.05 ug/ml).
Detection of 4EBP1 by Western Blot. Samples: Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody: Affinity purified rabbit anti-4EBP1 used at 0.25 ug/ml. Detection: Chemiluminescence.

EIF4EBP1 / 4EBP1 Antibody LS-C500901
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Human
ELISA IF IHC
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human pancreatic cancer tissue using EIF4EBP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human melanoma tissue using EIF4EBP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer tissue using EIF4EBP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human melanoma tissue using EIF4EBP1 Antibody at dilution of 1:100

EIF4EBP1 / 4EBP1 Antibody (C-Terminus) LS-C286408
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Rabbit, Mouse, Human
IF IHC IHC-P
Unconjugated
100µl  $415.00
Detection of Human 4EBP1 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:250.
Detection of Human 4EBP1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:250.
Detection of Human 4EBP1 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:250.
Detection of Human 4EBP1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:250.

EIF4EBP1 / 4EBP1 Antibody (10-90 aa) LS-C381832
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Rat, Human
ELISA IF IHC
Unconjugated
100µl 1mg/ml  $265.00

EIF4EBP1 / 4EBP1 Antibody (10-90 aa) LS-C380602
Rabbit Polyclonal (IgG) to Mouse EIF4EBP1 / 4EBP1
Mouse, Rat
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00

EIF4EBP1 / 4EBP1 Antibody LS-C400531
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human esophagus cancer using EIF4EBP1 Polyclonal Antibody at dilution of 1:50.
Immunohistochemistry of Human colon cancer using EIF4EBP1 Polyclonal Antibody at dilution of 1:50.
Immunohistochemistry of Human esophagus cancer using EIF4EBP1 Polyclonal Antibody at dilution of 1:50.
Immunohistochemistry of Human colon cancer using EIF4EBP1 Polyclonal Antibody at dilution of 1:50.

EIF4EBP1 / 4EBP1 Antibody LS-C403798
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human esophagus cancer using EIF4EBP1 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human ovarian cancer using EIF4EBP1 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human esophagus cancer using EIF4EBP1 Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human ovarian cancer using EIF4EBP1 Polyclonal Antibody at dilution of 1:30.

EIF4EBP1 / 4EBP1 Antibody (10-90 aa) LS-C381828
Rabbit Polyclonal (IgG) to Mouse EIF4EBP1 / 4EBP1
Mouse, Rat, Monkey
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of 4E-BP1 antibody
Western blot of 4E-BP1 antibody

EIF4EBP1 / 4EBP1 Antibody (phospho-Thr45) LS-C117284
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is blocked with the phospho peptide.

EIF4EBP1 / 4EBP1 Antibody (phospho-Thr36) LS-C117283
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr36) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 30', using 4E-BP1 (Phospho-Thr36) Antibody. The lane on the left is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr36) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 30', using 4E-BP1 (Phospho-Thr36) Antibody. The lane on the left is blocked with the phospho peptide.
Showing 1-25 of 157 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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