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Catalog Number Size Price
LS-C204512-100 100 µl (1 mg/ml) $376 
DCX / Doublecortin Antibody - Rat brain neural cultures stained with DCX / Doublecortin antibody (green), chicken polyclonal antibody to GFAP (red) and DNA (blue). The DCX / Doublecortin antibody antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for DCX / Doublecortin antibody. This DCX antibody is therefore an excellent marker of developing neuronal cells.
DCX / Doublecortin Antibody - Blots of crude rat brain extract from a postnatal 3 day animal stained with DCX / Doublecortin antibody. Two bands at ~45 kDa and ~35 kDa show that DCX / Doublecortin antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.
DCX / Doublecortin Antibody - Rat brain neural cultures stained with DCX / Doublecortin antibody (green), chicken polyclonal antibody to GFAP (red) and DNA (blue). The DCX / Doublecortin antibody antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for DCX / Doublecortin antibody. This DCX antibody is therefore an excellent marker of developing neuronal cells.
DCX / Doublecortin Antibody - Blots of crude rat brain extract from a postnatal 3 day animal stained with DCX / Doublecortin antibody. Two bands at ~45 kDa and ~35 kDa show that DCX / Doublecortin antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.
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Monoclonal Mouse anti‑Human DCX / Doublecortin Antibody (clone 3E1, IF, WB) LS‑C204512

Monoclonal Mouse anti‑Human DCX / Doublecortin Antibody (clone 3E1, IF, WB) LS‑C204512

Note: This antibody replaces LS-C180199
Antibody:
DCX / Doublecortin Mouse anti-Human Monoclonal (3E1) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204512-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DCX / Doublecortin Mouse anti-Human Monoclonal (3E1) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Doublecortin antibody LS-C204512 is an unconjugated mouse monoclonal antibody to Doublecortin (DCX) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
Target
Human DCX / Doublecortin
Synonyms
DCX | DBCN | Doublecortin | Doublin | Doublecortex | Lis-X | SCLH | LISX | XLIS | Lissencephalin-X
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
3E1
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant full length human Lis-A isoform of DCX purified from E. coli.
Specificity
Clone 3E1 is known to react with Fox3/NeuN from human, cow, pig, mouse, rat and other mammals. Since Fox3/NeuN is highly conserved, it is likely that the antibody is effective on other species also.
Applications
  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)
Usage
The antibody solution can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
Presentation
PBS, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DCX / Doublecortin
O43602 NM_000555 NP_000546.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/19/2024