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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DCX / Doublecortin

doublecortin

DCX / Doublecortin is a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: doublecortin
Synonyms: DCX, DBCN, Doublecortin, Doublin, Doublecortex, Lis-X, SCLH, LISX, XLIS, Lissencephalin-X
Target Sequences: NM_000555 NP_000546.2 O43602

Filtered By: None
Target
DCX / Doublecortin (59)
Reactivity
Human (47)
Mouse (36)
Rat (19)
Bat (1)
Bovine (3)
Chicken (4)
Chimpanzee (6)
Dog (1)
Drosophila (1)
Gibbon (6)
Hamster (1)
Horse (5)
Monkey (6)
Pig (2)
Rabbit (1)
Application
IHC (26)
IHC-Fr (1)
IHC-P (5)
WB (52)
Flo (6)
ELISA (22)
ICC (4)
IF (12)
IP (1)
Peptide-ELISA (6)
Host
rabbit (48)
mouse (7)
goat (4)
Isotype
IgG (43)
IgG1 (2)
IgG1,k (1)
IgG2a (1)
IgG2a,k (1)
Clonality
monoclonal mc (6)
polyclonal pc (53)
Clone
1G12 (1)
2G5 (1)
3E1 (1)
4A3 (1)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (7)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (7)
HRP Conjugated (6)
PE Conjugated (2)
Unconjugated (32)
Epitope
aa1-366 (10)
Ser128 (8)
aa254-303 (4)
aa362-411 (4)
180-260 aa (1)
Amino Acids 69-81 (1)
Lys292 (1)
aa206-255 (1)
aa232-242 (1)
aa284-297 (1)
aa346-395 (1)
aa413-417 (1)
internal region (1)
Publications
No (59)
Showing 1-25 of 59 results

DCX / Doublecortin Antibody (clone 2G5) IHC-plus™ LS-B8946
Mouse Monoclonal [clone 2G5] (IgG1) to Human DCX / Doublecortin
Human
ELISA Flo IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-DCX / Doublecortin antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunofluorescence of HepG2 cells using DCX mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Anti-DCX / Doublecortin antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunofluorescence of HepG2 cells using DCX mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

DCX / Doublecortin Antibody LS-C36204
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Drosophila, Rat, Mouse, Human, Monkey
ELISA Flo IF IHC IHC-Fr IP WB
Unconjugated
100µl  $530.00

DCX / Doublecortin Antibody (aa232-242) LS-C139686
Goat Polyclonal to Human DCX / Doublecortin
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IF IHC Peptide-ELISA WB
Unconjugated
100µg 0.5mg/ml  $405.00
Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

DCX / Doublecortin Antibody (clone 3E1) LS-C204512
Mouse Monoclonal [clone 3E1] (IgG2a,k) to Human DCX / Doublecortin
Mouse, Bovine, Rat, Pig, Human
ICC IF WB
Unconjugated
100µl 1mg/ml  $285.00
Rat brain neural cultures stained with DCX / Doublecortin antibody (green), chicken polyclonal antibody to GFAP (red) and DNA (blue). The DCX / Doublecortin antibody antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for DCX / Doublecortin antibody. This DCX antibody is therefore an excellent marker of developing neuronal cells.
Blots of crude rat brain extract from a postnatal 3 day animal stained with DCX / Doublecortin antibody. Two bands at ~45 kDa and ~35 kDa show that DCX / Doublecortin antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.
Rat brain neural cultures stained with DCX / Doublecortin antibody (green), chicken polyclonal antibody to GFAP (red) and DNA (blue). The DCX / Doublecortin antibody antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for DCX / Doublecortin antibody. This DCX antibody is therefore an excellent marker of developing neuronal cells.
Blots of crude rat brain extract from a postnatal 3 day animal stained with DCX / Doublecortin antibody. Two bands at ~45 kDa and ~35 kDa show that DCX / Doublecortin antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.

DCX / Doublecortin Antibody (aa284-297) LS-C155045
Goat Polyclonal to Mouse DCX / Doublecortin
Mouse, Rat, Human
IF Peptide-ELISA WB
Unconjugated
100µg 0.5mg/ml  $405.00
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
DCX antibody (0.5 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
DCX antibody (0.5 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DCX / Doublecortin Antibody LS-C203112
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
ELISA Flo IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $325.00
Immunohistochemistry of human brain tissue slide (Paraffin embedded) using Rabbit Anti-Doublecortin Polyclonal Antibody. and Purified Rabbit IgG (Whole molecule) Control (Right).
Western blot of tissue lysates using Doublecortin Antibody, pAb, Rabbit (1 ug/ml). The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 49 kD. Observed Size: 49 kD.
Immunohistochemistry of human brain tissue slide (Paraffin embedded) using Rabbit Anti-Doublecortin Polyclonal Antibody. and Purified Rabbit IgG (Whole molecule) Control (Right).
Western blot of tissue lysates using Doublecortin Antibody, pAb, Rabbit (1 ug/ml). The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 49 kD. Observed Size: 49 kD.

DCX / Doublecortin Antibody (clone 4A3) LS-C172397
Mouse Monoclonal [clone 4A3] (IgG1) to Human DCX / Doublecortin
Human
Flo IF WB
Unconjugated
100µl 0.73mg/ml  $345.00
Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.
Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.

DCX / Doublecortin Antibody LS-C485830
Mouse Monoclonal to Human DCX / Doublecortin
Human
Flo ICC IF
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

DCX / Doublecortin Antibody (Amino Acids 69-81) LS-C758313
Goat Polyclonal to Human DCX / Doublecortin
Mouse, Human
Flo IF Peptide-ELISA
Unconjugated
100µg  $405.00
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

DCX / Doublecortin Antibody (clone 1G12) LS-C197029
Mouse Monoclonal [clone 1G12] (IgG1,k) to Human DCX / Doublecortin
Human
ELISA IF WB
Unconjugated
100µg  $355.00
Immunofluorescence of monoclonal antibody to DCX on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of DCX expression in transfected 293T cell line by DCX monoclonal antibody (M01), clone 1G12.Lane 1: DCX transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of monoclonal antibody to DCX on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of DCX expression in transfected 293T cell line by DCX monoclonal antibody (M01), clone 1G12.Lane 1: DCX transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.

DCX / Doublecortin Antibody (Ser128) LS-C327218
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC WB
Unconjugated
200µl 0.25mg/ml  $530.00

DCX / Doublecortin Antibody (Ser128, Biotin) LS-C276860
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

DCX / Doublecortin Antibody (Ser128, FITC) LS-C276861
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

DCX / Doublecortin Antibody (Ser128, APC) LS-C276859
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

DCX / Doublecortin Antibody (Ser128, Azide-free, HRP) LS-C276862
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

DCX / Doublecortin Antibody (Ser128, AP) LS-C276858
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

DCX / Doublecortin Antibody (Ser128, PE) LS-C276863
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

DCX / Doublecortin Antibody LS-C752097
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC WB
Unconjugated
100µg  $335.00

DCX / Doublecortin Antibody (180-260 aa) LS-C383201
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Doublecortin antibody
Western blot of Doublecortin antibody
Western blot of Doublecortin antibody
Western blot of Doublecortin antibody

DCX / Doublecortin Antibody (internal region) LS-C742403
Goat Polyclonal to Mouse DCX / Doublecortin
Mouse
IF Peptide-ELISA WB
Unconjugated
100µg  $405.00
Antibody Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (
Antibody (0.003µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Antibody Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (
Antibody (0.003µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

DCX / Doublecortin Antibody LS-C396250
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
ELISA IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human liver tissue using antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human liver tissue using antibody at dilution of 1:100.

DCX / Doublecortin Antibody (aa1-366) LS-C372972
Rabbit Polyclonal (IgG) to Mouse DCX / Doublecortin
Mouse
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00; 10ml 0.5mg/ml  $2,160.00
Western blot of DCX / Doublecortin antibody.
Western blot of DCX / Doublecortin antibody.

DCX / Doublecortin Antibody (Ser128) LS-C97243
Rabbit Polyclonal to Human DCX / Doublecortin
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DCX Antibody (S128) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of lysate from human brain tissue lysate, using Phospho-DCX-S128. control. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DCX Antibody (S128) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of lysate from human brain tissue lysate, using Phospho-DCX-S128. control. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

DCX / Doublecortin Antibody (Lys292) LS-C176506
Rabbit Polyclonal to Human DCX / Doublecortin
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of Doublecortin (K292) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of Doublecortin (K292) pAb in extracts from Raw264.7 cells.
IHC of Doublecortin (K292) pAb in paraffin-embedded human lung carcinoma tissue.
Western blot of Doublecortin (K292) pAb in extracts from Raw264.7 cells.

DCX / Doublecortin Antibody LS-C749610
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human
IF WB
Unconjugated
50µl  $235.00
Immunofluorescence analysis of HeLa cells using DCX antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using DCX antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Showing 1-25 of 59 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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