IP-10 antibody LS-C663049 is an unconjugated mouse monoclonal antibody to human IP-10 (CXCL10). Validated for Flow, IF, IHC, RIA and WB.
Human CXCL10 / IP-10
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Frozen
- Western blot
- Flow Cytometry
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CXCL10 / IP-10 antibody was raised against recombinant human IP-10
FC: Antibody 6D4 stains human CXCL10. For intracellular staining activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation (Ref.3) W: Tissue was homogenized in loading sample buffer and subjected to SDS-PAGE. The band size is ~9 kDa (Ref.4). IHC-F: Tissue sections were fixed 10 minutes in acetone and pretreated with avidin/biotin blocking kit. As positive control chronically inflamed liver was used and as negative control normal liver and isotype matched antibody (Ref.4).
Store at 4°C for up to 1 year.
For research use only.