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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CXCL10 / IP‑10 Antibody LS‑C104367
Note: This antibody replaces LS-C54152, LS-C16065
IP-10 antibody LS-C104367 is an unconjugated rabbit polyclonal antibody to human IP-10 (CXCL10). Validated for ELISA and WB.
Catalog
Size
Price
LS-C104367-50
50 µg
$285
LS-C104367-100
100 µg
$325
Description
IP-10 antibody LS-C104367 is an unconjugated rabbit polyclonal antibody to human IP-10 (CXCL10). Validated for ELISA and WB.
Target
Human CXCL10 / IP-10
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CXCL10 / IP-10 antibody was raised against e.coli derived recombinant Human IP-10.
Specificity
Human IP-10
Usage
Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IP-10 (100 ng/ml), a concentration of 5-8 ug/ml of this antibody is required. ELISA: To detect hIP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IP-10 (LS-C104616) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIP-10. Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About CXCL10 / IP-10
P02778 NM_001565 NP_001556.2

Popular CXCL10 / IP-10 Products

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot, ELISA
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: Western blot, ELISA
Antibody
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Applications: Western blot, ELISA
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

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Images

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/18/2018
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